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Glycosylated haemoglobin and plasma glycoprotein assays by affinity chromatography

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Summary

A rapid chromatography method for separating glycosylated protein from non-glycosylated protein, using a boronate-agarose affinity medium which selectively binds the cis-diol groups of glycoproteins, was used to quantitate plasma glycoprotein as well as glycohaemoglobin. The results were found to be independent of: (1) temperature from 16.5 to 29.8 °C; (2) haemoglobin variants, and (3) aldimine glycoprotein adducts. Thus several of the common problems occurring in existing haemoglobin a1 assays are eliminated. There was a close correlation between glycohaemoglobin measured by affinity chromatography and haemoglobin A1 by cation-exchange (r = 0.959, n = 215). Specimens from 103 diabetic patients and 112 healthy volunteers were assayed. The following reference ranges were established: glycohaemoglobin 5.5–8.4%; glycosylated total protein 11.5–16.2%; glycoalbumin 11.6–19.5%.

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Willey, D.G., Rosenthal, M.A. & Caldwell, S. Glycosylated haemoglobin and plasma glycoprotein assays by affinity chromatography. Diabetologia 27, 56–58 (1984). https://doi.org/10.1007/BF00253503

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Key words

  • Glycohaemoglobin
  • plasma glycoprotein
  • affinity chromatography
  • diabetes
  • boronate-agarose resin