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European Journal of Nuclear Medicine

, Volume 1, Issue 1, pp 37–48 | Cite as

Specificity of immunoassays

I. Effect of plasma proteins on the specificity of steroid immunoassay
  • J. J. Pratt
  • W. Koops
  • M. G. Woldring
  • T. Wiegman
Article

Abstract

The theory of the measurement of the specificity of antigen binding by antibodies is reviewed. The specificity of steroid immunoassay has been investigated using a new solid phase radioimmunoassay system. The effects of plasma proteins on the specificity of the immunoassays have been tested. The methods used and the conclusions reached have been tested with homogeneous antibodies in order to simplify the interpretation of the results obtained. A new criterium of antibody homogeneity is proposed. The importance of correcting all results accurately for “non-specific binding” is emphasised. A new method for plotting radioimmunoassay standard curves is presented.

Keywords

Public Health Steroid Nuclear Medicine Plasma Protein Standard Curf 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • J. J. Pratt
    • 1
  • W. Koops
    • 1
  • M. G. Woldring
    • 1
  • T. Wiegman
    • 1
  1. 1.IsotopenlaboratoriumAcademisch ZiekenhuisGroningenThe Netherlands

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