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Gamma camera emission tomography using radiolabelled antibodies


Single photon emission computed tomography (SPECT) of 52 patients injected with 131I and 111In labelled monoclonal antibodies has been undertaken. Patients with tumors within the thorax or lower abdomen were imaged 48–72h following administration of 80 MBq 131I or 111In labelled monoclonal antibody. Tumour detection from the reconstructed views was compared with that from the planar images. Studies of patients injected with 131I labelled antibodies required long data collection times otherwise image quality was unsatisfactory. Emission tomography using 111In labelled antibodies, provided high quality reconstructed images using data collection times acceptable for most patients, and tumour deposits not confidently detected on planar images were clearly visualised in the axial views. These studies demonstrate the potential of radiolabelled antibody emission tomography.

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  1. Armitage NC, Perkins AC, Pimm HV, Farrands PA, Baldwin RW, Hardcastle JD (1984) The localisation of an anti-tumour monoclonal antibody (791T/36) in gastrointestinal tumours. Br J Surg 71:407–412

  2. Berche C, Mach J-P, Lumbroso J-D, Langlais C, Aubry F, Bucheggar F, Carrel S, Rougier P, Parmentier C, Tubiana M (1982) Tomoscintigraphy for detecting gastrointestinal and medullary thyroid cancers: first clinical results using radiolabelled monoclonal antibodies against carcinoembryonic antigen. Br Med J 285:1447–1451

  3. Clarke LP, Saw CB, Leong LK, Serafini AN (1985) SPECT imaging of 131I (364 keV): importance of collimation. Nucl Med Commun 6:41–47

  4. DeLand FH, Kim EE, Simmons G, Goldenberg DM (1980) Imaging approach in radioimmundetection. Cancer Res 40:3046–3049

  5. Epenetos AA, Britton KE, Mather S, Shepherd, J, Granowska M, Taylor-Papadimitriou J, Nimmon CC, Durbin H, Hawkins LR, Malpas JS, Bodmer WF (1982) Targetting of iodine-123-labelled tumour-associated monoclonal antibodies to ovarian, breast and gastrointestinal tumours. Lancet II:999–1004

  6. Farrands PA, Perkins AC, Pimm MV, Hardy JG, Baldwin RW, Hardcastle JD (1982) Radioimmunodetection of human colorectal cancers using an anti-tumour monoclonal antibody. The Lancet II:397–400

  7. Green AJ, Begent RHJ, Keep PA, Bagshawe KD (1984) Analysis of radioimmunodetection of tumours by the subtraction technique. J Nucl Med 25:96–100

  8. Hospital Physicists' Association (1983) Quality Control of Nuclear Instrumentation CRS-38

  9. Jarritt PH, Ell PJ (1984) Gamma camera emission computed tomography. Quality control and clinical applications. Current Medical Literature Ltd, London

  10. Jaszczak RJ, Coleman RE, Whitehead FR (1981) Physical factors affecting quantitative measurements using camera-based single photon emission computed tomography (SPECT). IEEE Transact Nucl Sci 28:69–80

  11. Jaszczak RJ, Whitehead FR, Kim CB, Coleman RE (1982) Lesion detection with single photon emission computed tomography (SPECT) compared with conventional imaging. J Nucl Med 23:97–102

  12. Keenan AM, Harbert JC, Larson SM (1985) Monoclonal antibodies in nuclear medicine. J Nucl Med 26:531–537

  13. Keyes WI (1981) Emission tomography. In: Moores BM, Parker RP, Pullan BR (eds) Physical aspects of medical imaging. Wiley, Chichester, pp 217–226

  14. Larson SM, Brown JP, Wright PW, Carrasquillo JA, Hellstrom I, Hellstrom KE (1983) Imaging of melanoma with I-131-labelled monoclonal antibodies. J Nucl Med 24:123–129

  15. Larsson SA (1980) Gamma camera emission tomography. Acta Radiol [Suppl] 363:1–75

  16. Lentle BC, Scott JR, Schmidt RP, Hooper HR, Catz Z (1985) The clinical value of direct tumour scintigraphy: A new hypothesis. J Nucl Med 26:1215–1217

  17. Mach J-P, Buchegger F, Forini M, Ritschard J, Berche C, Lumbross J-D, Mchrege M, Giradet C, Accolla RS, Carrel, S (1981) Use of radiolabelled monoclonal anti-CEA antibodies for the detection of human carcinomas by external photoscanning and tomoscintigraphy. Immunol Today 2:239–249

  18. Ott RJ, Grey LJ, Zivanovic MA, Flower MA, Trott NG, Moshakis V, Coombes RC, Omerod MG, Westwood JH, McCready VR (1983) The limitations of the dual radionuclide subtraction technique for the external detection of tumours by radioiodinelabelled antibodies. Br J Radiol 56:101–108

  19. Perkins AC, Whalley DR, Hardy JG (1984) Physical approach for the reduction of, dual radionuclide image subtraction artefacts in immunoscintigraphy. Nucl Med Commun 5:501–512

  20. Perkins AC, Pimm MV, Birch MK (1985) The preparation and characterization of 111In-labelled 791T/36 monoclonal antibody for tumour immunoscintigraphy. Eur J Nucl Med 10:296–301

  21. Pimm MV, Embleton MJ, Perkins AC, Price MR, Robins RA, Robinson GR, Baldwin RW (1982) In vivo localisation of antiosteogenic carcoma 791T monoclonal antibody in osteogenic sarcoma xenografts. Int J Cancer 30:75–85

  22. Symonds EM, Perkins AC, Pimm MV, Baldwin RW, Hardy JG, Williams DA (1985) Clinical implications for immunoscintigraphy in patients with ovarian malignancy: A preliminary study using monoclonal antibody 791T/36. Br J Obstet Gynaecol 92:270–276

  23. Williams MR, Perkins AC, Campbell FC, Pimm MV, Hardy JG, Wastie ML, Blamey RW, Baldwin RW (1984) The use of monoclonal antibody 791T/36 in the immunoscintigraphy of primary and metastatic carcinoma of the breast. Clin Oncol 10:375–381

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Correspondence to Alan C. Perkins.

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Perkins, A.C., Whalley, D.R., Ballantyne, K.C. et al. Gamma camera emission tomography using radiolabelled antibodies. Eur J Nucl Med 14, 45–49 (1988).

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Key words

  • Radiolabelled antibody
  • Emission tomography
  • Tumour detection