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Isolation and characterization of a 2,4-dichlorophenoxyacetic acid-degrading soil bacterium

Summary

A 2,4-dichlorophenoxyacetic acid (2,4-D)-degrading bacterial strain, Xanthobacter sp. CP, was isolated after enrichment in aerated soil columns. A limited number of chlorinated phenols and chlorinated phenoxyalkanoic acids with an even number of carbon atoms in the side chain served as substrates for growth, although whole cells exhibited oxygen uptake with a wide range of those compounds. The maximal growth rate with 2,4-D was 0.13·h-1 at a growth yield of 0.1 g biomass/g 2,4-D. Chloride ions were released quantitatively from 2,4-D and related chlorinated aromatic compounds which served as growth substrates. No by-products of 2,4-D metabolism were detected in oxygen-sufficient cultures of Xanthobacter sp. CP and catechols were cleaved exclusively by catechol 1,2-dioxygenase.

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Correspondence to Franz Streichsbier.

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Ditzelmüller, G., Loidl, M. & Streichsbier, F. Isolation and characterization of a 2,4-dichlorophenoxyacetic acid-degrading soil bacterium. Appl Microbiol Biotechnol 31, 93–96 (1989). https://doi.org/10.1007/BF00252535

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Keywords

  • Chloride
  • Phenol
  • Chlorinate
  • Carbon Atom
  • Bacterial Strain