An automatic feeding system to supply olive oil in semi-batch culture was established by monitoring cell concentration with a laser turbidimeter combined with a microcomputer and a pulse motor. In this automatic feeding system, specific olive oil supply rate (g olive oil) · (g dry cell)-1 · h-1, q 0, was changed in an appropriate range. Attempts were made to produce lipase by a turbidity-dependent automatic fed-batch culture of Pseudomonas fluorescens. It was found from the semi-batch cultures with turbidity-dependent feeding of olive oil and with varied initial Fe ion concentrations that excess Fe ion was inhibitory to formation of the lipase. Turbidity-dependent automatic simultaneous feeding of olive oil and Fe ion was performed to obtain semi-deficiencies of both the oily substrate in the culture liquid and Fe content of the cells. Using this semi-batch culture, high lipase activity, 5600 units/ml, was attained at an optimal value of q 0.
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Ishihara, K., Suzuki, T., Yamane, T. et al. Effective production of Pseudomonas fluorescens lipase by semi-batch culture with turbidity-dependent automatic feeding of both olive oil and iron ion. Appl Microbiol Biotechnol 31, 45–48 (1989). https://doi.org/10.1007/BF00252524
- Lipase Activity
- Pseudomonas Fluorescens
- Supply Rate
- Oily Substrate