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Effect of alcohols and temperature on the hopanoid content of Zymomonas mobilis

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Summary

The influence of different culture conditions on the hopanoid content of Zymomonas mobilis was investigated in batch cultures. With a gas-liquid chromatographic method it could be shown that the content of 1,2,3,4-tetrahydroxypentane-29-hopane (THBH) reached a maximum value in the stationary phase due to the high level of ethanol accumulated in the medium. The hopanoid content increased sharply with the addition of ethanol to the culture. Ethanol was shown to be the most effective of the alcohols tested in causing an increase of the hopanoid content. Furthermore, an alteration of the incubation temperature from 14° to 37°C also caused an increase of the amount of hopanoids.

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Abbreviations

THBH:

1,2,3,4-tetrahydroxy-pentane-29-hopane

HMG-CoA reductase:

3-hydroxy-3-methylglutaryl coenzyme A reductase

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Correspondence to Andrea Schmidt.

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Schmidt, A., Bringer-Meyer, S., Poralla, K. et al. Effect of alcohols and temperature on the hopanoid content of Zymomonas mobilis . Appl Microbiol Biotechnol 25, 32–36 (1986). https://doi.org/10.1007/BF00252509

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Keywords

  • Alcohol
  • Culture Condition
  • Stationary Phase
  • Batch Culture
  • Chromatographic Method