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Factors affecting growth physiology of Bacillus thuringiensis

Summary

Physiological studies on Bacillus thuringiensis var. entomocidus revealed the failure of the organism to survive or sporulate under low aeration levels, notably in the presence of high sugar concentrations. Cell counts, sporulation titers and potency of resulting endotoxin were found to vary with the level of aeration. The incremental feeding of glucose with continuous pH adjustment prevented cell injury and death which results from prolonged exposure to acidity liberated at the high sugar concentrations which occur when glucose is added batchwise. Increasing of dipotassium phosphate concentration in growth medium increased the potency of the resulting endotoxin.

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References

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Correspondence to H. S. Salama.

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Foda, M.S., Salama, H.S. & Selim, M. Factors affecting growth physiology of Bacillus thuringiensis . Appl Microbiol Biotechnol 22, 50–52 (1985). https://doi.org/10.1007/BF00252156

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Keywords

  • Acidity
  • Bacillus
  • Aeration
  • Prolonged Exposure
  • Cell Injury