Living Aspergillus niger cells were entrapped in polyacrylamide gels and employed in both replacement batch and continuous column bioreactors to produce citric acid from sucrose. With the replacement batch bioreactor, increase of citric acid was observed under conditions of higher aeration and of wider surface of immobilized cells. With the continuous bioreactor, the maximum citric acid yield was 39.1 mg/h per 40 g gels. The biocatalyst activity or half-life was 105 days.
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Horitsu, H., Adachi, S., Takahashi, Y. et al. Production of citric acid by Aspergillus niger immobilized in polyacrylamide gels. Appl Microbiol Biotechnol 22, 8–12 (1985). https://doi.org/10.1007/BF00252149
- Citric Acid
- Aspergillus Niger
- Immobilize Cell