Advertisement

Springer Nature is making Coronavirus research free. View research | View latest news | Sign up for updates

Application of material and energy balances in the interpretation of intermediate product build-up in batch growth of Pseudomonas aeruginosa on n-hexadecane

  • 27 Accesses

Summary

This work is concerned with the application of material and energy balances in an attempt to understand the phenomenon of product build-up when Pseudomonas aeruginosa is grown on n-hexadecane in a batch fermentor. It is shown that the organism accumulates a polyactide, called poly-B-hydroxybutyrate (PHB) during early stages of growth and metabolizes it at later stages of growth. This explains the low carbon and available electron balances which have been observed.

This is a preview of subscription content, log in to check access.

Abbreviations

d :

Moles of carbon dioxide per quantity of organic substrate containing one g atom carbon, g mole/g atom carbon

m e :

Rate of organic substrate consumption for maintenance, g equiv. of available electrons/g equiv. of available electrons in biomass (h)

\(Q_{{\text{CO}}_{\text{2}} }\) :

Specific rate of evolution of carbon dioxide, g moles/g dry wt (h)

\(Q_{{\text{O}}_{\text{2}} }\) :

Specific rate of oxygen consumption, g mole/g dry wt (h)

s :

Organic substrate concentration, g/liter

t :

Time (h)

x :

Biomass concentration, g/liter

y c :

Biomass carbon yield (fraction of organic substrate carbon in biomass), dimensionless

γ b :

Reductance degree of biomass, equivalents of available electrons per g atom carbon

γ s :

Reductance degree of substrate, equivalents of available electrons per g atom carbon

ɛ:

Fraction of energy in organic substrate which is evolved as heat, dimensionless

η:

Fraction of energy in organic substrate which is coverted to biomass or biomass energetic yield, dimensionless

μ:

Specific growth rate, h-1

σ b :

Weight fraction carbon in biomass, dimensionless

σ s :

Weight fraction carbon in substrate, dimensionless

References

  1. Brisbane PG, Ladd JN (1965) Therole of micro-organisms in Petroleum exploration. Annu Rev Microbiol 19:351

  2. Erickson LE, Minkevich IG, Eroshin VK (1978) Application on mass and energy balances regularities in fermentation. Biotechnol Bioeng 20:1595–1621

  3. Erickson LE, Selga SE, Viesturs UE (1978) Application of mass and energy balance regularities to products formation. Biotechnol Bioeng 20:1623–1638

  4. Erickson LE, Minkevich IG, Viesturs UE (1979) Utilization of mass-energy balance regularities in the analysis of continuous culture data. Biotechnol Bioeng 21:575–591

  5. Erickson LE, Hess JI (1981) Analysis of growth and polysaccharide yields in chemostat cultures of Rhizobium trifolii. Annals of NY Academy of Science 369:81–90

  6. Ferrer A, Erickson LE (1980) Data consistency, yield, maintenance and hysteresis in batch cultures of Candida lipolytica cultured on n-hexadecane. Biotechnol Bioeng 22:421–450

  7. Foster JWA (1962) Hydrocarbons as substrates for micro-organisms. Antonie Van Leeuwenkiek J Microbiol Serol 28:241

  8. Frank LH, DeMoss RD (1959) On the biosynthesis of pyocyanine. J Bacteriol 77:776–782

  9. Hildebrand FB (1956) Introduction to numerical analysis. McGraw-Hill, New York, Toronto, London 64–68

  10. Ingledew WM, Campbell JJR (1969) A new resuspension medium for pyocyanine production. Can J Microbiol 15:595–598

  11. Layokun SK (1978) Hydrocarbons as fermentation feedstocks. A paper presented at SPE/AIME symposium, PTI, Warri, July 7

  12. Layokun SK (1982) Growth of Pseudomonas aeruginosa on n-hexadecane. J Nigerian Soc Chemical Engr 1:69–82

  13. Louca A (1970) The Growth of Pseudomonas aeruginosa NC1B 9904 on n-hexadecane. Ph. D. Thesis, University of London

  14. McKenna EJ, Kallio RE (1965) The biology of hydrocarbons. Ann Rev Microbiol 19:183

  15. Solomon BO, Erickson LE, Hess JL (1981) Application of data consistency tests and new parameter estimation methods to microbial growth on corn dust in batch culture. Biotechnol Bioengr 23:2333–2360

  16. Solomon BO, Erickson LE (1981) Biomass yields and maintenance requirements for growth on carbonhydrates. Proc Biochem 16:44–49

  17. Solomon BO, Erickson LE, Hess JE, Yang SS (1982) Maximum likelihood estimation of growth yields. Biotechnol Bioeng 24:633–649

  18. Whooley MA, McLoughlin AJ (1982) The regulation of pyocyanine production in Pseudomonas aeruginosa. European J Appl Microbiol Biotechnol 15:161–166

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Layokun, S.K., Solomon, B.O. & Fatile, I.A. Application of material and energy balances in the interpretation of intermediate product build-up in batch growth of Pseudomonas aeruginosa on n-hexadecane. Appl Microbiol Biotechnol 22, 255–258 (1985). https://doi.org/10.1007/BF00252026

Download citation

Keywords

  • Pseudomonas
  • Energy Balance
  • Pseudomonas Aeruginosa
  • Intermediate Product
  • Electron Balance