Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Photoproduction of amino acids by mutant strains of N2-fixing cyanobacteria

  • 32 Accesses

  • 10 Citations


Mutant strains of the N2-fixing cyanobacterium bacterium Anabaena variabilis resistant to 6-fluorotryptophan or to ethionine were isolated. Many of these strains liberated amino acids into their media in the absence of 6-fluorotryptophan and ethionine. Nitrogenase activity was higher in mutant strains than in the parent strain. Mutant strains were immobilised in calcium alginate and sustained photoproduction of amino acids has been demonstrated.

This is a preview of subscription content, log in to check access.







4-(2-hydroxyethyl)-1, piperazine ethanesulphonic acid




3-deoxy-d-arabinoheptulosonate 7-phosphate

chl a:

chlorophyll a


  1. Alix J (1982) Molecular aspects of the in vivo and in vitro effects of ethionine, an analog of methionine. Microbiol Rev 46:281–295

  2. Antarikanonda P (1984) Production of extracellular amino acids by cyanobacterium Anabaena siamensis Antarikanonda. Current Microbiol 11:191–196

  3. Bloom FR, Kretschner PJ (1983) In: Wise DL (ed) Organic Chemicals from Biomass, Benjamin/Cummings Publishing Co Ltd pp 145–169

  4. Clement Y, Escoffier B, Trombe MC, Laneelle G (1984) Is glutamate excreted by its uptake system in Corynebacterium glutamicum? A working hypothesis. J Gen Microbiol 130:2589–2594

  5. Fogg GE (1952) The production of extracellular nitrogenous substances by a blue-green alga. Proc Roy Soc B Lond 139:372–397

  6. Fogg GE, Stewart WDP, Fay P, Walsby AE (1973) The Blue-Green Algae. Academic Press London

  7. Hall G, Flick MB, Jensen RA (1980) Approach to recognition of regulatory mutants of cyanobacteria. J Bacteriol 143:981–988

  8. Jensen RA, Hall GC (1982) Endo-oriented control of pyrimidally arranged metabolic branchpoints. Trends Biochem Sci 7:177–185

  9. Kerby NW, Musgrave SC, Codd GA, Rowell P, Stewart WDP (1983) Photoproduction of ammonia by immobilized cyanobacteria. Proceedings: Biotech '83. Online Publications Ltd, Northwood, UK, pp 1029–1036

  10. Kerby NW, Rowell P, Stewart WDP (1985) Ethylenediamine uptake and metabolism in the cyanobacterium Anabaena variabilis. Arch Microbiol 141:244–248

  11. Kerby NW, Musgrave SC, Rowell P, Shestakov SV, Stewart WDP (1986) Photoproduction of ammonium by immobilised mutant strains of cyanobacteria. Appl Microbiol Biotechnol 24:42–26

  12. Mackinney G (1941) Absorption of light by chlorophyll solutions. J Biol Chem 140:315–322

  13. Miflin BJ, Lea PJ (1977) Amino acid metabolism. Ann Rev Plant Physiol 28:299–329

  14. Musgrave SC, Kerby NW, Codd GA, Stewart WDP (1982) Sustained ammonia production by immobilized filaments of the nitrogen fixing cyanobacterium Anabaena 27893. Biotechnol Letts 4:647–652

  15. Musgrave SC, Kerby NW, Codd GA, Stewart WDP (1983a) Structural features of calcium alginate entrapped cyanobacteria modified for ammonia production. Eur J Appl Microbiol Biotechnol 17:133–136

  16. Musgrave SC, Kerby NW, Codd GA, Rowell P, Stewart WDP (1983b) Reactor types for the utilization of immobilized photosynthetic microorganisms. Process Biochem (Suppl) 184–190

  17. Polukhina LE, Sakhurieva GN, Shestakov SV (1982) Ethylenediamine-resistant Anabaena variabilis mutants with derepressed nitrogen-fixing system. Microbiology 51:90–95

  18. Riccardi G, Silvio S, Cifferri O (1981) Production of amino acids by analogue resistant mutants of the cyanobacterium Spirulina platensis. J Bacteriol 147:1002–1007

  19. Rippka R, Deruelles J, Waterbury JB, Herdman M, Stanier RY (1979) Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J Gen Microbiol 111:1–61

  20. Rosen H (1957) A modified ninhydrin colorimetric analysis for amino acids. Arch Biochem Biophys 67:10–15

  21. Sakhurieva GN, Polukhina LE, Shestakov SV (1982) Glutamine synthesis in Anabaena variabilis mutants with derepressed nitrogenase. Microbiology 51:308–312

  22. Stewart WDP (1963) Liberation of extracellular nitrogen by two nitrogen-fixing blue-green algae. Nature 200:1020–1021

  23. Stewart WDP (1980) Some aspects of structure and function in N2-fixing cyanobacteria. Ann Rev. Microbiol 34:497–536

  24. Stewart WDP (1987) The functional organization of nitrogen-fixing cyanobacteria. Phil Trans Roy Soc Lond B (in press)

  25. Stewart WDP, Fitzgerald, GP, Burris RH (1967) In situ studies on N2-fixation using the acetylene reduction technique. Proc Natl Acad Sci USA 58:2071–2078

  26. Yamada K, Kinoshita S, Tsunoda T, Aida K (1972) The Microbial Production of Amino Acids. Kodansha International Ltd, Tokyo

Download references

Author information

Correspondence to William D. P. Stewart.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Kerby, N.W., Niven, G.W., Rowell, P. et al. Photoproduction of amino acids by mutant strains of N2-fixing cyanobacteria. Appl Microbiol Biotechnol 25, 547–552 (1987). https://doi.org/10.1007/BF00252015

Download citation


  • Calcium
  • Alginate
  • Mutant Strain
  • Parent Strain
  • Anabaena