The localization of 111In-labelled MA5 monoclonal antibody, reactive with a breast tumor associated antigen, was studied in 17 patients. MA5 was selected because 1) it reacts with >95% of primary and metastatic lesions, 2) the recognized antigen is present on the cell surface in vivo and 3) MA5 gives excellent localization in human breast tumor xenografts. Each patient received 2 mg antibody labeled with 5 mCi 111In and in some cases, 3 mg or 18 mg unlabeled carrier antibody. No serious allergic reactions were noted. There was a large uptake in the liver, less significant uptake in the spleen and bone, and minimal accumulation in the bowel. Bone lesions, primary tumors, soft tissue recurrences and lung metastases larger than 3 cm diameter were imaged, while only 1 lesion smaller than 3 cm was detected. Non specific accumulation of tracer was noted at the site of a port-a-cath, in a hematoma, in fibrocystic lesions, and at sites of previous radiation treatment. Extensive fibrosis and poor vascularization characteristic of breast tumors may explain in part the limited sensitivy of the imaging.
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Abdel-Nabi HH, Schwartz AN, Higano CS, Wechter DG, Unger NW (1987) Colorectal carcinoma: detection with Indium-111 anti-carcinoembryonic antigen monoclonal antibody ZCE-025. Radiology 164:617–622
Berche C, Mach JP, Lombroso JD, Langlais C, Aubry F, Buchegger F, Carrel S, Rougier P, Parmentier C, Tubiana M (1982) Tomascintigraphy for detecting gastrointestinal and medullary thyroid cancers: first clinical results using radiolabeled monoclonal antibodies against carcinoembryonic antigen. Br Med J 285:1447–1451
Capone PM, Papsidero LD, Chu TM (1984) Relationship between antigen density and immunotherapeutic response elicited by monoclonal antibodies against solid tumors. JNCI 72:673–677
Davies JO, Davies ER, Howe K, Jackson PC, Pitcher EM, Sadowski CS, Stirrat GM, Sunderland CA (1985) Radionuclide imaging of ovarian tumors with 123I-labeled monoclonal antibody (NDOG2) directed against placental alkaline phosphatase. Br J Obstet Gynecol 92:277–286
Dion AS, Major PP, Ishida M (1987) Characterization of the cross related antigens of human milk fat globule membrane and breast tumors detected by a new monoclonal antibody panel. In: Ceriani RL (ed) Immunological approaches to the diagnosis and therapy of breast cancer. New York Plenum, New York, pp 3–19
Engelstad BL, Spitler LE, Del Rio MJ, Ramos EC, Rosendorf LL, Reinhold CE, Khentigan A, Huberty JP, Corpuz SW, Lee HM, Okerlund MD, Hattner RS, Scannon PJ (1986) Phase 1 immunolymphoscintigraphy with an In-111 labeled anti-melanoma monoclonal antibody. Radiology 161:419–422
Epenetos AA, Britton KE, Mather S, Shepherd J, Granowska M, Taylor-Papdimitriou J, Nimmon CC, Duoban H, Hawkins LR, Malpas JS, Bodmer WF (1982) Targeting of iodine-123-labelled tumuor-associated monoclonal antibodies to ovarian, breast and gastrointestinal tumors. Lancet II (8306):999–1005
Goldenberg DM, DeLand F, Kim E, Bennett S, Primus FJ, van Nagell JR, Estes N, DeSimone P, Rayburn P (1978) Use of radiolabeled antibodies to carcinoembryonic antigen for the detection and localization of diverse cancers by external photo scanning. New Engl J Med 298:1384–1388
Goodwin DA (1987) Pharmacokinetics and antibodies. J Nucl Med 28:1358–1362
Griffiths AA, Padmanabhan N, Shepherd P, Lazarus C, Maisey M, Fentiman I, Rubens R, Taylor-Papadimitriou J (1986) Monoclonal antibody HMFG-2 retains activity in vivo and binds to high molecular weight components expressed by metastatic breast cancers. Mol Biol Med 3:425–435
Hayes DF, Zalustsky MR, Kaplan W, Noska M, Thor M, Colcher D, Kufe DW (1986) Pharmacokinetics of radiolabeled monoclonal antibody B6.2 in patients with metastatic breast cancer. Cancer Res 46:3157–3163
Mach JP, Carrel S, Forni M, Ritschard J, Donath A, Alberto P (1980) Tumor localization of radiolabeled antibodies against carcinoembryonic antigen in patients with carcinoma. New Engl J Med 303:5–10
Mach JP, Chatal JF, Lumbroso JD, Buchegger F, Forni M, Ritschard J, Berche C, Douillard JY, Carrel S, Herlyn M (1983) Tumor localization in patients by radiolabeled monoclonal antibodies agains colon carcinoma. Cancer Res 43:5593–6000
Major PP, Kovac PE, Lavallée ML, Kovalik EC (1987a) Monoclonal antibodies to antigens abnormally expressed in breast cancer. J Histochem Cytochem 35:139–148
Major PP, Lavallée M, Minassian H, Kovac P, Wang N-S (1987b) Ultra structural localization of a breast tumor associated antigen. J Histochem Cytochem 35:375–379
Mann BD, Cohen MB, Saxton RE, Morton DL, Benedict WF, Korn EL, Spolter L, Graham LS, Chang CC, Burk M (1984) Imaging of human tumor xenografts in nude mice with radiolabeled monoclonal antibodies. Cancer 54:1318–1327
Murray JL, Rosenblum MG, Lamki L, Glenn HJ, Krizan Z, Hersch EM, Plager CE, Bartholomew RM, Unger MW, Carlo DJ (1987) Clinical parameters related to optimal tumor localization of Indium-111-labeled mouse anti-melanoma monoclonal antibody ZME-018. J Nucl Med 28:25–33
Nelp WB, Eary JF, Jones RF, Hellstrom KE, Hellstrom I, Beaumier PL (1987) Preliminary studies of monoclonal antibody lymphoscintigraphy in malignant melanoma. J Nucl Med 28:34–41
Rainsbury RM, Westwood JH, Coombes RC, Neville AM, Ott RJ, Kalirai TS, McCready VR, Gazet JC (1983) Localization of metastatic breast carcinoma by a monoclonal antibody chelate labeled with Indium-111. Lancet 2:934–938
Rainsbury R (1984) Localization of human breast carcinoma by radiolabeled monoclonal antibodies. Br J Surg 71:805–812
Ryan KP, Dillman RO, DeNardo SJ, DeNardo GL, Beauregard J, Hagan PL, Amox DG, Clutter ML, Sobol RE, Abramson I, Bartholomew RK, Frincke JM, Birdwell CR, Carlo DJ, O'Grady LF, Halpern SE, Burnett KG, Rulot CM (1988) Breast cancer imaging with In-111 human 1gM monoclonal antibodies: Preliminary Studies. Radiology 167:71–75
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Major, P., Wang, T., Ishida, M. et al. Breast cancer imaging with mouse monoclonal antibodies. Eur J Nucl Med 15, 655–660 (1989). https://doi.org/10.1007/BF00251679
- Monoclonal antibodies radioimmunoscanning