Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

The comparison of 8-hydroxyquinoline, tropolone, and acetylacetone as mediators in the labelling of polymorphonuclear leucocytes with indium-111: A functional study

  • 60 Accesses

  • 14 Citations


Tropolone forms a lipophilic complex with indium-111 which is capable of mediating the labelling of polymorphonuclear leucocytes (PMNs) by this isotope; labelling efficiencies are comparable with the best achieved using 8-hydroxyquinoline and acetylacetone. However, in terms of PMN chemotaxis and phagocytosis, tropolone is significantly less toxic than either of the other ligands. 8-Hydroxyquinoline was found to reduce PMN chemotaxis and phagocytosis to approximately 70% of the control values at a concentration of 20 μM. Tropolone may prove a superior labelling reagent.

This is a preview of subscription content, log in to check access.


  1. Addison IE, Babbage JW (1975) A raft technique for chemotaxis: a versatile method suitable for clinical studies. J Immunol Methods 10:385–388

  2. Doherty PW, Buchberg JT, Lipton MJ, Meares CF, Goodwin DA (1978) The use of indium-111 labeled leucocytes for abscess detection. Clin Nucl Med 3:108–111

  3. Holley TR, Van Eppes DE, Harvey RL (1974) Effect of high doses of radiation on human neutrophil chomotaxis, phagocytosis and morphology. Am J Pathol 75:61–72

  4. Martin WR, Gurevich N, Goris ML, McDougall IR (1979) Detection of occult abscesses with 111In-labelled leucocytes. AJR 133:123–125

  5. McAfee JG, Thakur ML (1976) Survey of radioactive agents for in vitro labeling of phagocytic leucocytes. 1. Soluble agents. J Nucl Med 17:480–487

  6. Mustafa MG, Cross CE, Tyler WS (1971) Interference of Cadmium ion with oxidative metabolism of alveolar macrophages. Arch Intern Med 127:1050–1058

  7. Penny R, Galton DAG, Scott JT, Eisen V (1966) Studies on neutrophil function. 1. Physiological and pharmacological aspects. Br J Haematol 12:623–632

  8. Segal AW, Arnot RN, Thakur HL, Lavender JP (1976) Indium-111 labeled leucocytes for localisation of abscesses. Lancet 2:1056–1058

  9. Segal AW, Deteix P, Garcia R, Tooth P, Zanelli GD, Allison AC (1978) Indium-111 labeling of leucocytes: a detrimental effect on neutrophil and lymphocyte function and an improved method of cell labeling. J Nucl Med 19:1238–1244

  10. Thakur ML, Choleman RE, Welch MJ (1976) Indium-111 labeled leucocytes for the localisation of abscesses: preparation, analysis, tissue distribution and comparison with gallium-67 citrate in dogs. J Lab Clin Med 89:217–228

  11. Thakur ML, Lavender JP, Arnot RN, Silvester DJ, Segal AW (1977) Indium-111 labeled autologous leucocytes in man. J Nucl Med 18:1012–1019

  12. Tuck DG (1966) Studies in the coordination chemistry of indium-111. Coord Chem Rev 1:286

  13. Zahkireh B, Thakur ML, Malech HL, Cohen MS, Gottschalk A, Root RK (1979) Indium-111 labeled human polymorphonuclear leucocytes: viability, random migration, chemotaxis, bactericidal capacity and ultrastructure. J Nucl Med 20:741–747

Download references

Author information

Correspondence to Paul Wyeth.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Burke, J.E.T., Roath, S., Ackery, D. et al. The comparison of 8-hydroxyquinoline, tropolone, and acetylacetone as mediators in the labelling of polymorphonuclear leucocytes with indium-111: A functional study. Eur J Nucl Med 7, 73–76 (1982).

Download citation


  • Public Health
  • Nuclear Medicine
  • Functional Study
  • Acetylacetone
  • Labelling Efficiency