The use of region-of-interest (ROI) techniques to quantify data obtained in radionuclide images is common-place. However, the reproducibility of quantitation due to inter- and intra-observer variations using particular methods of deriving ROIs is often not appreciated. We examined such variations in the results obtained by four independent observers of varying experience using four methods of depicting a ROI about an organ. The set of image data consisted of renal scans with varying target-to-background ratios, and the ROI facilities included two edge-detection methods. The results indicated that, once observers were experienced with edge-detection methods, a lower inter- and intra-observer variation could be achieved, although the technique of ‘shrinking’ a ROI about a subjectively chosen display level was reasonably satisfactory. In terms or reproducibility, the least satisfactory method of depicting a ROI was the commonly used manually guided ‘bug’ around arbitarily chosen display levels representing the boundary of an organ.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Biello DR, Sampathkumaran KS, Geltman EM, Britson WA, Scott DJ, Grbac RT (1981) Determination of left ventricular ejection fraction: A new method that requires minimal operator training. J Nucl Med Technol 9:77–80
Bourguignon MH, Douglass KH, Links JM, Wagner HN Jr. (1981) Fully automated data acquisition, processing and display in equilibrium radioventriculography. Eur J Nucl Med 6:343–347
Davis LS (1975) A survey of edge detection techniques. Comput Graphics Image Process 4:248–270
Gandsman EJ, North DL, Schulman RS, Bough EN (1981) Measurement of the ventricular stroke volume ratio by gated radionuclide angiography. Radiology 138:161–165
Goris ML, McKillop JH, Briandet PA (1981) A fully automated determination of the left ventricular region of interest in nuclear angiocardiography. Cardiovasc Intervent Radiol 4:117–123
Jackson PC (1982) Evaluating the performance of an edge detection algorithm for use in radioisotope imaging. Phys Med Biol 27:1045–1055
Jackson PC, Wilde P, Watt I, Davies ER (1981) An edge detection algorithm for use in radionuclide imaging. Eur J Nucl Med 6:33–38
Pizer SM, Todd-Pokropek AE (1978) Improvement of scintigrams by computer processing. Semin Nucl Med 2:125–146
Shutsky R, Pfisterer M, Verba J, Battler A, Ashburn W (1980) Influence of different background and left ventricular assignments on the ejection fraction in equilibrium radionuclide angiography. Radiology 135:725–730
Taylor DN, Garvie NW, Harris D, Sharrantt GP, Goddard BA, Ackery DM (1980) The effect of various background protocols on the measurement of left ventricular ejection fraction in equilibrium radionuclide angiography. Br J Radiol 53:205–209
About this article
Cite this article
Jackson, P.C., Jones, M., Brimble, C.E. et al. The reduction of inter- and intra-observer variability for defining regions of interest in nuclear medicine. Eur J Nucl Med 11, 186–189 (1985). https://doi.org/10.1007/BF00251372
- Region of interest
- Edge detection