Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Different effects of hypothermia on insulin and glucagon secretion from the isolated perfused rat pancreas

  • 107 Accesses

  • 7 Citations


Two series of experiments with the isolated perfused rat pancreas were performed in parallel. The conditions differed only with respect to temperature, which was 37.5 °C in one series and 28 °C in the other. The lowering of the temperature decreased insulin secretion induced by glucose as well as the insulin response to tolbutamide and acetylcholine. Unlike insulin, glucagon secretion was not significantly modified by hypothermia. Our results suggest that the mechanisms involved in glucagon and insulin secretion are different.


  1. 1.

    Ashcroft SJH, Chatra L, Weerasinghe C, Randle PJ (1973) Interrelationship of islet metabolism, adenosine triphosphate content and insulin release. Biochem J 132: 223–231

  2. 2.

    Coore HG, Randle PJ (1964) Regulation of insulin secretion studied with pieces of rabbit pancreas incubated in vitro. Biochem J 93: 66–75

  3. 3.

    Curry DL, Curry KP (1970) Hypothermia and insulin secretion. Endocrinology 87: 750–755

  4. 4.

    Gerich JE, Charles MA, Grodsky GM (1974) Characterization of the effects of arginine and glucose on glucagon and insulin release from the perfused rat pancreas. J Clin Invest 54: 833–841

  5. 5.

    Gerich JE, Frankel BJ, Fanska R, West L, Forsham PH, Grodsky GM (1974) Calcium dependency of glucagon secretion from the in vitro perfused rat pancreas. Endocrinology 94: 1381–1385

  6. 6.

    Grodsky GM, Batts AA, Bennett LL, Vcella C, McWilliams NB, Smith DF (1963) Effects of carbohydrates on secretion of insulin from isolated rat pancreas. Am J Physiol 205: 638–644

  7. 7.

    Hahn HJ, Ziegler M (1977) Investigations on isolated islets of Langerhans in vitro. 16. Modification of the glucose-dependent inhibition of glucagon secretion. Biochim Biophys Acta 499: 362–372

  8. 8.

    Hales CN, Randle PJ (1963) Immunoassay of insulin with insulin-antibody precipitate. Biochem J 88: 137–146

  9. 9.

    Iversen J, Hermansen K (1977) Calcium, glucose and glucagon release. Diabetologia 13: 297–303

  10. 10.

    Kikuchi M, Wolheim CB, Cuendet GS, Renold AE, Sharp GWG (1978) Studies on the dual effects of glucose on 45 Ca++ efflux from isolated rat islets. Endocrinology 102: 1339–1345

  11. 11.

    Loubatières A, Mariani MM, Ribes G, De Malbosc H, Chapal J (1969) Etude expérimentale d'un nouveau sulfamide hypoglycémiant particulièrement actif, le HB 419 ou glibenclamide. I. Action bêtacytotrope et insulino-sécrétrice. Diabetologia 5: 1–10

  12. 12.

    Matschinsky FM, Pagliara AS, Hover BA, Haymond MW, Stillings SN (1975) Differential effects of alpha- and beta-D-glucose on insulin and glucagon secretion from the isolated perfused rat pancreas. Diabetes 24: 369–372

  13. 13.

    Pradhan TK, Criss WE (1977) Temperature effects on the modulation of adenylate cyclases from rat liver and Morris hepatomas. Oncology 34: 258–260

  14. 14.

    Unger RH, Aguilar-Parada E, Müller WA, Eisentraut AM (1970) Study of pancreatic alpha cell function in normal and diabetic subjects. J Clin Invest 49: 837–848

Download references

Author information

Additional information

Equipe de Recherche Associée au CNRS n∘ 786

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Loubatières-Mariani, M.M., Chapal, J., Puech, R. et al. Different effects of hypothermia on insulin and glucagon secretion from the isolated perfused rat pancreas. Diabetologia 18, 329–333 (1980). https://doi.org/10.1007/BF00251015

Download citation

Key words

  • Hypothermia
  • temperature
  • insulin release
  • glucagon release
  • isolated perfused rat pancreas
  • glucose
  • acetylcholine
  • tolbutamide
  • arginine