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Use of protoplast fusion to introduce methionine overproduction into Saccharomyces cerevisiae

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Summary

dl-Ethionine-resistant mutants of Saccharomyces uvarum ATCC 26602 were found to overproduce exogenous l-methionine. dl-Ethionine-resistant mutant ER 108, carrying a mutation to chloramphenicol resistance was converted to petite form, and protoplasts obtained from it were fused with protoplasts from antibiotic-sensitive S. cerevisiae X2928 carrying six auxotrophies. The resulting fusants maintained four auxotrophies and were capable of overproducing l-methionine. These fusants were stable after ten passages on complete medium.

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References

  1. Barney MC, Jansen GP, Helbert JR (1980) Use of spheroplast fusion and genetic transformation to introduce dextrin utilization into Saccharomyces uvarum. J Am Soc Brew Chem 38:1–5

  2. Bortol A, Nudel C, Fraile E de Torres R, Giulietti A, Spencer JFT, Spencer D (1986) Isolation of yeast with killer activity and its breeding with an industrial baking strain by protoplast fusion. Appl Microbiol Biotechnol 24:414–416

  3. Dunyak SA, Cook TM (1985) Continuous fermenter growth of a methionine-overproducing mutant of Candida utilis. Appl Microbiol Biotechnol 21:182–186

  4. Farahnak F, Seki T, Ryu DDY, Ogrydziak D (1986) Construction of lactose-assimilating and high-ethanol-producing yeasts by protoplast fusion. Appl Environ Microbiol 51:362–367

  5. Komatsu K, Yamada T, Kodaira R (1974) Isolation and characteristics of pool methionine-rich mutants of a Candida sp. J Ferm Technol 52:93–99

  6. Kreger-van Rij NJW (ed) (1984) The yeasts. A taxonomic study. Elsevier Scientific Publications, Amsterdam

  7. Legmann R, Margalith P (1986) Ethanol formation by hybrid yeasts. Appl Microbiol Biotechnol 23:198–202

  8. Morzycka E, Sawnor-Korsynska D, Paszewski A, Grabski J, Raczynska-Bojanowska K (1976) Methionine overproduction by Saccharomycopsis lipolytica. Appl Environ Microbiol 32:125–130

  9. Okanishi M, Gregory KF (1970) Isolation of mutants of Candida tropicalis with increased methionine content. Can J Microbiol 16:1139–1143

  10. Pina A, Calderon IL, Benitez T (1986) Intergeneric hybrids of Saccharomyces cerevisiae and Zygosaccharomyces fermentati obtained by protoplast fusion. Appl Environ Microbiol 51:995–1003

  11. Putrament A, Baranowska H, Prazmo W (1973) Induction by manganese of mitochondrial antibiotic resistance mutations in yeast. Mol Gen Genet 126:357–366

  12. Slonimski PP, Perrodin G, Croft JH, (1968) Ethidium bromide induced mutation of yeast mitochondria: complete transformation of cells into respiratory deficient non-chromosomal “petites”. Biochem Biophys Res Commun 30:232–239

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Correspondence to Diego Matteuzzi.

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Brigidi, P., Matteuzzi, D. & Fava, F. Use of protoplast fusion to introduce methionine overproduction into Saccharomyces cerevisiae . Appl Microbiol Biotechnol 28, 268–271 (1988). https://doi.org/10.1007/BF00250453

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Keywords

  • Methionine
  • Saccharomyces Cerevisiae
  • Chloramphenicol
  • Saccharomyces
  • Complete Medium