Strains degrading 3-methylbenzoate (3MB) via ortho-cleavage were enriched by preselection with 4-carboxymethyl-2-methylbut-2-en-1,4-olide (2-methyllactone, 2ML) as sole carbon source or by counter selection of meta-cleaving strains using 3-chlorobenzoate (3CB) as suicide substrate. These strains and microorganisms obtained from continuous cultures with 3CB/3MB (Schmidt et al. 1985) or with chlorophenols and cresols (Schmidt 1987) were grouped according to their abilities to grow on 3CB, 3MB or 2ML and their mode of ring-cleavage during growth with aromatic substrates. Each group was tested for its capability to mineralize mixtures of 3CB and 3MB and the extent of DOC-removal was quantified.
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Taeger, K., Knackmuss, H. & Schmidt, E. Biodegradability of mixtures of chloro- and methylsubstituted aromatics: Simultaneous degradation of 3-chlorobenzoate and 3-methylbenzoate. Appl Microbiol Biotechnol 28, 603–608 (1988). https://doi.org/10.1007/BF00250420
- Carbon Source
- Sole Carbon Source
- Continuous Culture