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Archives of Microbiology

, Volume 159, Issue 2, pp 189–196 | Cite as

Spectroscopic and potentiometric characterization of cytochromes in two Sporomusa species and their expression during growth on selected substrates

  • Beate Kamlage
  • Andreas Boelter
  • Michael Blaut
Original Papers

Abstract

The homoacetogenic bacteria Sporomusa ovata and Sporomusa sphaeroides were grown on betaine, betaine + formate, and acetoin in the absence of carbon dioxide, and the formation of membrane-bound cytochromes was determined. In S. sphaeroides, the growth substrate had little influence on the expression of cytochromes. In contrast, membranes from betaine-or acetoin-grown S. ovata cells had an 11-or 3-fold higher cytochrome b content than cells grown on betaine + formate. The cytochrome c content was reduced below the detection level after growth on the latter two substrates. The cytochromes in the membranes of S. sphaeroides and S. ovata were characterized by low-temperature difference spectroscopy, hemochrome difference spectroscopy, and redox potentiometry. Membranes of S. ovata were shown to contain two b-type cytochromes with Em,7=-153±10 mV and Em,7=-226±14 mV and two c-type cytochromes with Em,7=-86±6 mV and Em,7=-265±10 mV. In S. sphaeroides also two b-type cytochromes with Em,7=-165±7 mV and Em,7=-241±2 mV and two c-type cytochromes with Em,7=-101±4 mV and Em, 8.5=-338±9 mV could be distinguished. Cell extracts of S. sphaeroides were shown to contain all the enzymes of the acetyl-CoA (Wood) pathway. The degradation pathways of the substrates tested and the possible role of the cytochromes are discussed.

Key words

Cytochromes Redox difference spectroscopy Midpoint potential Betaine Formate Acetoin Acetyl-CoA pathway Sporomusa ovata Sporomusa sphaeroides 

Abbreviations

Em,7

midpoint potential at pH 7 and 25°C

H4F

tetrahydrofolate

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Beate Kamlage
    • 1
  • Andreas Boelter
    • 1
  • Michael Blaut
    • 1
  1. 1.Institut für Mikrobiologie der Universität GöttingenGöttingenFederal Republic of Germany

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