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Properties of the β-d-glucosidase (cellobiase) from the wood-rotting fungus, Coriolus versicolor

Summary

Structural and kinetic parameters of the β-d-glucosidase (cellobiase, β-d-glucoside glucohydrolase) from Coriolus versicolor have been determined. It is a high molecular weight glycoprotein (300,000 d) composed 10% by weight of protein, 90% by weight of carbohydrate in which glucose is the primary hexose sugar. The Km for 4-nitrophenyl-β-d-glucopyranoside (4 NPG) and cellobiose are 0.276 and 2.94 mM respectively at pH 4.5 and 40°. d-Glucose is a competitive inhibitor with a Ki of 1.8 mM with 4 NPG as substrate, and at high concentrations, cellobiose exhibits a substrate inhibition effect on the enzyme, so negating attempts to overcome the competitive inhibition of glucose by increasing the concentration of the substrate.

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Correspondence to Christine S. Evans.

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Evans, C.S. Properties of the β-d-glucosidase (cellobiase) from the wood-rotting fungus, Coriolus versicolor . Appl Microbiol Biotechnol 22, 128–131 (1985). https://doi.org/10.1007/BF00250032

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Keywords

  • Glucose
  • Enzyme
  • Sugar
  • Molecular Weight
  • Carbohydrate