Structural and kinetic parameters of the β-d-glucosidase (cellobiase, β-d-glucoside glucohydrolase) from Coriolus versicolor have been determined. It is a high molecular weight glycoprotein (300,000 d) composed 10% by weight of protein, 90% by weight of carbohydrate in which glucose is the primary hexose sugar. The Km for 4-nitrophenyl-β-d-glucopyranoside (4 NPG) and cellobiose are 0.276 and 2.94 mM respectively at pH 4.5 and 40°. d-Glucose is a competitive inhibitor with a Ki of 1.8 mM with 4 NPG as substrate, and at high concentrations, cellobiose exhibits a substrate inhibition effect on the enzyme, so negating attempts to overcome the competitive inhibition of glucose by increasing the concentration of the substrate.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Abrams E (1948) Microbiological deterioration of organic materials its prevention and methods of test. National Bureau of Standards Misc. Publ. No. 188 U.S. Dept. of Commerce, Washington
Albersheim P, Nevins DJ, English PD, Karr A (1967) A method for the analysis of sugars in plant cell-wall polysaccharides by gas-liquid chromatography. Carbohydrate Res 5: 340–345
Bergheim LER, Pettersson LG, Axiö-Fredriksson U-B (1975) Mechanism of Enzymatic cellulose degradation — characterization and enzymatic properties of a β-1, 4-glucan cellobiohydrolase from Trichoderma viride. Eur J Biochem 53: 55–62
Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1976) Colorimetric method for determination of sugars and related substances. Anal Chem 28: 350–356
Eriksson KE (1978) Enzyme Mechanisms involved in cellulose hydrolysis by the rot fungus Sporotrichum pulverulentum. Biotech Bioeng 20: 317–332
Evans CS, Farmer JY, Palmer JM (1984) An extracellular haem-protein from Coriolus versicolor. Phytochemistry 23: 1247–1250
Ishihara T (1980) Lignin Biodegradation: Microbiology, chemistry and potential applications. Vol. 11, 17–31. CRC Press, Florida
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275
Pettersson G, Porath J (1963) Studies on cellulolytic enzymes. II. Multiplicity of the cellulolytic enzymes of Polyporus versicolor. Biochim Biophys Acta 67: 9–15
Pettersson G, Cowling EB, Porath J (1963) Studies on cellulolytic enzymes. I. Isolation of a low molecular weight cellulase from Polyporus versicolor. Biochim Biophys Acta 67: 1–8
Shewale JG (1982) β-Glucosidase: its role in cellulase synthesis and hydrolysis of cellulose. Int J Biochem 14: 435–443
Woodward J, Wiseman A (1982) Fungal and other β-d-glucosidases—their properties and applications. Enz Microbiol Technol 4: 73–79
About this article
Cite this article
Evans, C.S. Properties of the β-d-glucosidase (cellobiase) from the wood-rotting fungus, Coriolus versicolor . Appl Microbiol Biotechnol 22, 128–131 (1985). https://doi.org/10.1007/BF00250032
- Molecular Weight