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The periplasmic nitrite reductase of Thauera selenatis may catalyze the reduction of selenite to elemental selenium

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Abstract

Thauera selenatis grows anaerobically with selenate, nitrate or nitrite as the terminal electron acceptor; use of selenite as an electron acceptor does not support growth. When grown with selenate, the product was selenite; very little of the selenite was further reduced to elemental selenium. When grown in the presence of both selenate and nitrate both electron acceptors were reduced concomitantly; selenite formed during selenate respiration was further reduced to elemental selenium. Mutants lacking the periplasmic nitrite reductase activity were unable to reduce either nitrite or selenite. Mutants possessing higher activity of nitrite reductase than the wild-type, reduced nitrite and selenite more rapidly than the wild-type. Apparently, the nitrite reductase (or a component of the nitrite respiratory system) is involved in catalyzing the reduction of selenite to elemental selenium while also reducing nitrite. While periplasmic cytochrome C 551 may be a component of the nitrite respiratory system, the level of this cytochrome was essentially the same in mutant and wild-type cells grown under two different growth conditions (i.e. with either selenate or selenate plus nitrate as the terminal electron acceptors). The ability of certain other denitrifying and nitrate respiring bacteria to reduce selenite will also be described.

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References

  1. Brown NW, Watkinson JH (1977) An automated method for the determination of nanogram quantities of selenium. Anal Chim Acta 89: 29–35

  2. Burton GA, Giddings TH, DeBrine P, Fall R (1987) High incidence of selenite-resistant bacteria from a site polluted with selenium. Appl Environ Microbiol 53: 185–188

  3. Clesceri LS (ed) (1989) Standard methods for the examination of water and wastewater, 17th edn. American Public Health Association, Washington, DC

  4. Coyne MS, Tiedje JA (1990) Distribution and diversity of dissimilatory nitrite reductases in denitrifying bacteria. In: Revsbech NP, Sorensen J (eds) FEMS Symposium 56: Denitrification in soil and sediment. Plenum, New York, pp 21–35

  5. Doran JW (1982) Microorganisms and the biological cycling of selenium. Adv Microb Ecol 6: 1–32

  6. Macy JM, Michel TA, Kirsch DG (1989) Selenate reduction by a Pseudomonas species: a new mode of anaerobic respiration. FEMS Microbiol Lett 61: 195–198

  7. Macy JM, Rech S, Auling G, Dorsch M, Stackebrandt E, Sly L (1993) Thauera selenatis gen. nov., sp. nov., a member of the beta-subclass of Proteobacteria with a novel type of anaerobic respiration. Int. J Syst Bacteriol 43: 135–142

  8. Markwell MAK, Haas SM, Bieber LL, Tolbert NE (1978) A modification of the Lowry procedure to simplify protein determination in membrane lipoprotein samples. Anal Biochem 87: 206–210

  9. Rech S, Macy JM (1992) The terminal reductases for selenate and nitrate respiration in Thauera selenatis are two different enzymes. J Bacteriol 174: 7316–7320

  10. Schmidt K, Jensen SL, Schlegel HG (1963) Die Carotinoide der Thiorhodaceae. Arch Mikrobiol 46: 117–126

  11. Schröder I, Roberton AM, Bokranz M, Unden G, Bocher R, Kröger A (1985) The membranous nitrite reductase involved in the electron transport of Wolinella succinogenes. Arch Microbiol 140: 380–386

  12. Shapleigh JP, Payne WJ (1985) Differentiation of c, d1 cytochrome and copper nitrite reductase production in denitrifiers. FEMS Microbiol Lett 26: 275–279

  13. Whetter PA, Ullray DE (1978) Improved method for determining selenium. J Assoc Off Anal Chem 61: 927–930

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DeMoll-Decker, H., Macy, J.M. The periplasmic nitrite reductase of Thauera selenatis may catalyze the reduction of selenite to elemental selenium. Arch. Microbiol. 160, 241–247 (1993). https://doi.org/10.1007/BF00249131

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Key words

  • Anaerobic respiration
  • Selenate
  • Nitrate
  • Selenite reduction
  • Nitrite reductase
  • Selenium (elemental) Thauera selenatis