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Archives of Microbiology

, Volume 156, Issue 3, pp 163–166 | Cite as

Production of a novel ω1-eicosapentaenoic acid by Mortierella alpina 1S-4 grown on 1-hexadecene

  • Sakayu Shimizu
  • Saeree Jareonkitmongkol
  • Hiroshi Kawashima
  • Kengo Akimoto
  • Hideaki Yamada
Original Papers

Abstract

An arachidonic-producing fungus, Mortierella alpina 1S-4, was found to accumulate ω-unsaturated fatty acids of C-20 chain length together with ω1-hexadecenoic acid, ω1-octadecenoic acid and so on, when grown on 1-alkenes, i.e., 1-hexadecene and 1-octadecene. The results of mass spectroscopy and proton NMR showed that a C20 polyunsaturated fatty acid (PUFA) is a novel cis-5,8,11,14,19-eicosapentaenoic acid (20:5ω1). This PUFA was obtained at a yield of 0.13 mg/ml culture broth (2.8% of the fungal total fatty acid content) on cultivation of the fungus in a medium containing 4% (v/v) 1-hexadecene and 1% yeast extract at 28°C for 1 week. Investigation of the distribution of fatty acids showed that about 90% (by mol.) of the PUFA was present in the triglycerides and 10% was in the phospholipid fraction. About 70% of that found in the phospholipids was phosphatidylcholine (PC) and the value accounted for ca. 10% of the total fatty acid content. The formation of these ω-unsaturated fatty acids was presumed to occur through the arachidonic acid biosynthetic pathway (n-6 route).

Key words

Fatty acid metabolism 1-Hexadecene ω1-Eicosapentaenoic acid Mortierella alpina 

Abbreviations

PUFA

polyunsaturated fatty acid

EPA

cis-5,8,11,14,17-eicosapentaenoic acid

TG

triglycerides

PS

phosphatidylserine

PC

phosphatidylcholine

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References

  1. Cerniglia CE, Perry JJ (1974) Effect of substrate on the fatty acid composition of hydrocarbon-utilizing filamentous fungi. J Bacteriol 118:844–847PubMedPubMedCentralGoogle Scholar
  2. Christie WW (1982) Lipid analysis, 2nd edn. Pergamon Press, Oxford, pp 93–106Google Scholar
  3. Folch J, Lees M, Sloane-Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497–509PubMedGoogle Scholar
  4. Klug MJ, Markovetz AJ (1967) Degradation of hydrocarbons by members of the genus Candida. III. Oxidative intermediates from 1-hexadecene and 1-heptadecene by Candida lipolytica. J Bacteriol 93:1847–1852PubMedPubMedCentralGoogle Scholar
  5. Shimizu S, Kawashima H, Shinmen Y, Akimoto K, Yamada H (1988a) Production of eicosapentaenoic acid by Mortierella fungi. J Am Oil Chem Soc 65:1455–1459CrossRefGoogle Scholar
  6. Shimizu S, Shinmen Y, Kawashima H, Akimoto K, Yamada H (1988b) Fungal mycelia as a novel source of eicosapentaenoic acid: activation of enzyme(s) involved in eicosapentaenoic acid production at low temperature. Biochem Biophys Res Commun 150:335–341CrossRefGoogle Scholar
  7. Shimizu S, Akimoto K, Kawashima H, Shinmen Y, Yamada H (1989a) Production of dihomo-γ-linolenic acid by Mortierella alpina 1S-4. J Am Oil Chem Soc 60:237–241CrossRefGoogle Scholar
  8. Shimizu S, Kawashima H, Shinmen Y, Akimoto K, Yamada H (1989b) Microbial conversion of an oil containing α-linolenic acid to an oil containing eicosapentaenoic acid. J Am Oil Chem Soc 66:342–347CrossRefGoogle Scholar
  9. Shinmen Y, Shimizu S, Akimoto K, Kawashima H, Yamada H (1989) Production of arachidonic acid by Mortierella fungi: selection of a potent producer and optimization of culture conditions for large-scale production. Appl Microbiol Biotechnol 31:11–16CrossRefGoogle Scholar
  10. Wada H, Murata N (1989) Synechocystis PCC6803 mutants defective in desaturation of fatty acid. Plant Cell Physiol 30:971–978CrossRefGoogle Scholar
  11. Yamada H, Shimizu S, Shinmen Y (1987) Production of arachidonic acid by Mortierella elongata 1S-5. Agric Biol Chem 51:785–790Google Scholar

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Sakayu Shimizu
    • 1
  • Saeree Jareonkitmongkol
    • 1
  • Hiroshi Kawashima
    • 1
  • Kengo Akimoto
    • 1
  • Hideaki Yamada
    • 1
  1. 1.Department of Agricultural Chemistry, Faculty of AgricultureKyoto UniversityKyotoJapan

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