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Archives of Microbiology

, Volume 159, Issue 6, pp 541–544 | Cite as

Endogenous respiration reflects the energy load imposed by transport of nonmetabolizable substrates and by induced de novo protein synthesis in Rhodotorula glutinis

  • S. Janda
  • K. Sigler
  • M. Höfer
Original Papers

Abstract

Uptake of the nonmetabolizable sugars 6-deoxy-d-glucose, l-rhamnose and l-xylose, which are taken up by a common carrier, stimulated significantly cell respiration in Rhodotorula glutinis. The extra oxygen consumption for uptake (0.5–0.7 equivalents O2/mol transported sugar) was proportional to the uptake rate and was independent of the Ktvalue of the transport system. Sugars that become metabolized after induction, d-arabinose and methyl-α-d-glucoside, caused a higher stimulation, 1.4 and 3.6 equivalents O2/mol respectively, which was reduced to 0.6 equivalents O2/mol when de novo protein synthesis was blocked by cycloheximide. The stimulation of respiration thus includes a fraction related purely to the energy demand for uptake and another one related to the induced de novo protein synthesis. The net uptake-induced respiration boost was similar with all sugars under study irrespective of their transport systems. The estimated energy demand was equivalent to about 2 ATP/sugar molecule. For comparison, the amino acid analogue α-aminoisobutyric acid (AIB) was also investigated; the overall energy demand for its uptake corresponded to the equivalent of about 4 ATP/molecule.

Key words

Substrate uptake Energy requirement Endogenous respiration Nonmetabolized substrate Induced catabolism Rhodotorula glutinis 

Abbreviation

AIB

α-aminoisobutyric acid

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • S. Janda
    • 1
  • K. Sigler
    • 1
  • M. Höfer
    • 2
  1. 1.Institute of MicrobiologyCzechoslovak Academy of SciencesPrague 4Czech Republic
  2. 2.Bolanisches Institut der Universität BonnBonn 1Germany

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