Nitrogen metabolism of Frankia strain, Cc01, Mg+, At4 and Hr18
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The composition and levels of amino acids in four Frankia strains isolated from different actinorhizal plants, were determined. Minor differences in the amino acid profiles were noted with GLN (GLU) being the major amino acid in all four strains. Enzyme actives of ammonia metabolism, GS (glutamine synthetase), GOGAT (glutamate synthetase), and GDH (glutamate dehydrogenase), were also measured. In strains At4 and Hr18, GS and GOGAT activity levels were elevated in N2-grown cells but significant amounts of GDH activity were present in ammonia-grown cells. No GDH was detected in strain Cc01 and Mg+. The characters of heat-stable and heat-labile GSs were described. In N2-fixing cells, the ATP and amino acid content was much lower, but ammonia content was higher than in NH inf4 sup+ -grown cells.
Key wordsFrankia Nitrogen metabolism Glutamine synthetase Glutamate synthetase Glutamate dehydrogenase Amino acid ATP Ammonia
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- Burris RH (1974) Methodology. In: Quispel A (ed) The biology of nitrogen fixation. North-Holland Amsterdam, pp 9–33Google Scholar
- Dixon POD, Wheeler CT (1986) Nitrogen fixation in plants. Chapman and Hall, New YorkGoogle Scholar
- Farnden KJF, Robertson JG (1980) Methods for studying enzymes involved in metabolism related to nitrogenase. In: Bergersen FJ (ed) Methods for evaluating biological nitrogen fixation, John Wiley & Sons, New York, pp 265–314Google Scholar
- Institute of Soil, Academia Sinica, Nanjing (1983) Analysis of soil physics and chemistry (in Chinese). Science Publishing House, Beijing, pp 81–84Google Scholar
- Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein determination with the Folin Phenol reagent. J Biol Chem 193: 265–275Google Scholar
- Pate JS, Atkins CA, Rainbird RM (1981) Theoretical and experimental costing of nitrogen fixation and related processes in nodules of legumes. In: Gibson AH, Newton WE (eds) Current perspectives in nitrogen fixation. Australian Academy of Science, pp 105–116Google Scholar
- Weiguang W (1982) Preparation and properties of crude luciferinluciferase system from firefly lantern. Plant Physiol Commun (Chinese) 92: 34–37Google Scholar
- Weiguang W, Jianben G (1986) Comparison of the methods for extracting ATP from plant leaves. Plant Physiol Commun (Chinese) 117: 54–56Google Scholar
- Yang W, Jian D (1987) Studies on biological characteristics of Frankia sp. from Hippophae rhamnoides. Acta Microbiologica Sinica 27: 227–232Google Scholar
- Zhongwei L, Jian D (1985) Isolation of Frankia sp. FSAt4 from root nodules of Alnus tinctoria Sarg. Cultivation in vitro and investigation on its infectivity. J Microbiol (Chinese) 5: 17–20Google Scholar
- Zhongwei L, Jian D (1986) Isolation of Frankia sp. FSCc01 from root nodules of Casuarina cunninghamiana and its cultivation in vitro and nodulation conditions. Acta Microbiologica Sinica 26: 295–301Google Scholar