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Archives of Microbiology

, Volume 159, Issue 6, pp 526–529 | Cite as

Cytochemical evidence of a fungal cell wall alteration during infection of Eucalyptus roots by the ectomycorrhizal fungus Cenococcum geophilum

  • Fabienne Paris
  • Jean Dexheimer
  • Frédéric Lapeyrie
Original Papers

Abstract

When the ectomycorrhizal fungus Cenococcum geophilum changes from a saprophytic to a symbiotic stage, its cell wall structure becomes simplified. The external hyphal wall layer which, in the saprophytic stage, is highly reactive to the Gomori-Swift test becomes poorly reactive and can no longer be distinguished from the internal wall layer in the Hartig net hyphae. The intensely stained external wall layer was also absent from pure cultures of Cenococcum geophilum grown on a medium with a low sugar content. This cell wall alteration could be due to a decrease in the amount of melanin or of melanin plus cystine-containing proteins. This change may be necessary for increased nutrient exchange between symbionts through hyphal walls.

Key words

Ectomycorrhiza Fungus Cell wall, ultrastructure protein melanin Eucalyptus Cenococcum geophilum 

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Fabienne Paris
    • 1
  • Jean Dexheimer
    • 2
  • Frédéric Lapeyrie
    • 1
  1. 1.Centre de Recherche de Nancy, Laboratoire de MicrobiologieINRAChampenouxFrance
  2. 2.Laboratoire de Biologie des LigneuxUniversité de Nancy IVandoeuvre CedexFrance

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