Cibacron blue 3G-A inhibits cell separation of gram-positive bacteria
- 35 Downloads
A triazine dye, Cibacron blue 3G-A (CB), is an inhibitor of cell separation of staphylococcal spp. therefore, we examined the effect of CB on growth of grampositive bacteria other than Staphylococcus. CB added to the medium of growing cultures of strains of genus Micrococcus, Streptococcus, Lactobacillus and Bacillus caused inhibition of cell separation. Moreover, in case of Bacillus and Lactobacillus, individual cells were elongated as filament. Strains of the genus Micrococcus were as sensitive to CB as genus Staphylococcus in which the minimum concentrations of CB needed for inhibition of cell separation ranged from 15 to 100 μM. Other strains belong to genus Streptococcus, Bacillus and Lactobacillus were less sensitive; the minimum concentrations were 100 μM–25 mM.
Key wordsCibacron blue Cell separation Chain Filament Cluster Micrococcus Streptococcus Bacillus Lactobacillus
Unable to display preview. Download preview PDF.
- MatsuhashiM, YamadaM, Hirose-KumagaiA, KoyamaT (1977) Formation of cell groupings in gram positive cocci. In: IshikawaT, MaruyamaY, MatsumiyaH (eds) Growth and differentiation in microorganisms. University of Tokyo Press, Tokyo, pp 59–76Google Scholar
- PerkinsHR (1980) The bacterial autolysins. In: RogersHJ, PerkinsHR, WardJB (eds) Microbial cell walls and membranes. Chapman and Hall, London, pp 437–456Google Scholar
- SugaiM, KoikeH, HongY-M, MiyakeY, NogamiR, SuginakaH (1989) Purification of a 51 kDa endo-β-N-acetylglucosaminidase from Staphylococcus aureus. FEMS Microbiol Lett 61:267–272Google Scholar
- WardJB, WilliamsonR (1984) Bacterial autolysins: specificity and function. In: NombelaC (ed) Microbial cell wall synthesis and autolysis. Elsevier, Amsterdam, pp 159–166Google Scholar