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Archives of Microbiology

, Volume 155, Issue 2, pp 153–158 | Cite as

Formation and role of glycine betaine in the moderate halophile Vibrio costicola

  • C. G. Choquet
  • I. Ahonkhai
  • M. Klein
  • D. J. Kushner
Original Papers

Abstract

The moderate halophile Vibrio costicola, growing on a chemically-defined medium, transformed choline into glycine betaine (betaine) by the membrane-bound enzyme choline dehydrogenase and the cytoplasmic enzyme betainal (betaine aldehyde) dehydrogenase. Choline dehydrogenase was strongly induced and betainal dehydrogenase less strongly induced by choline. The formation of these enzymes was also regulated by the NaCl concentration of the growth medium, increasing with increasing NaCl concentrations. Intracellular betaine concentrations also increased with increasing choline and NaCl concentrations in the medium. This increase was almost completely blocked by chloramphenicol, which does not block the increase in salt-tolerant active transport on transfer from a low to a high salt concentration.

Choline dehydrogenase was inhibited by chloride salts of Na+, K+, and NH inf4 su+ , the inhibition being due to the Cl- ions. Betainal dehydrogenase was stimulated by 0.5 M salts and could function in up to 2.0 M salts.

Cells grew as well in the presence as in the absence of choline in 0.5 M and 1.0 M NaCl, but formed no intracellular betaine. Choline stimulated growth in 2.0 M NaCl and was essential for growth in 3.0 M NaCl. Thus, while betaine is important for some of the adaptations to high salt concentration by V. costicola, it by no means accounts for all of them.

Key words

Halophilic Vibrio costicola Choline dehydrogenase Betaine aldehyde dehydrogenase Betainal dehydrogenase Glycine betaine Transport 

Abbreviations

CDMM

chemically-defined minimal medium

PPT

proteose-peptone tryptone medium

SDS

sodium dodecyl sulfate

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • C. G. Choquet
    • 1
  • I. Ahonkhai
    • 1
  • M. Klein
    • 1
  • D. J. Kushner
    • 1
  1. 1.Department of BiologyUniversity of OttawaOttawaCanada

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