Archives of Microbiology

, Volume 155, Issue 2, pp 143–148 | Cite as

Desulfovibrio alcoholovorans sp. nov., a sulfate-reducing bacterium able to grow on glycerol, 1,2- and 1,3-propanediol

  • A. I. Qatibi
  • V. Nivière
  • J. L. Garcia
Original Papers


Desulfovibrio strain SPSN was isolated from an anaerobic industrial fermenter fed with waste water from the alcohol industry. The isolate was a gram-negative, non-spore-forming, curved organism, the motility of which is provided by a single polar flagellum. The oxidation of substrates was incomplete and included glycerol and 1,3-propanediol. Sulfate, sulfite, thiosulfate, and sulfur were utilized as electron acceptors. Pyruvate, fumarate and malate could be fermented. The DNA base composition was 64.5±0.3% G+C. Cytochrome c3 and desulfoviridin were present. On the basis of these characteristics and because strain SPSN could not be ascribed to any of the existing species, the isolate is established as a new species of the genus Desulfovibrio, and the name Desulfovibrio alcoholovorans is proposed.

Key words

Sulfate reduction Fermentation Glycerol 1,2-Propanediol 1,3-Propanediol Desulfovibrio 


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  1. BraunM, StolpH (1985) Degradation of methanol by a sulfatereducing bacterium. Arch Microbiol 142:77–80CrossRefGoogle Scholar
  2. Cord-RuwischR (1985) A quick method for the determination of dissolved and precipitated sulfides in cultures of sulfate-reducing bacteria. J Microbiol Methods 4:33–36CrossRefGoogle Scholar
  3. EsnaultG, CaumetteP, GarciaJL (1988) Characterization of Desulfovibrio giganteus sp. nov., a sulfate-reducing bacterium isolated from a brackish coastal lagoon. Syst Appl Microbiol 10:147–151CrossRefGoogle Scholar
  4. HansenTA (1988) Physiology of sulfate-reducing bacteria. Microbiol Sci 5:81–84PubMedGoogle Scholar
  5. HungateRE (1960) Microbial ecology of rumen. Bacteriol Rev 24:353–364PubMedPubMedCentralGoogle Scholar
  6. Imhoff-StuckleD, PfennigN (1983) Isolation and characterization of a nicotinic acid-degrading sulfate-reducing bacterium, Desulfococcus niacini sp. nov. Arch Microbiol 136:194–198CrossRefGoogle Scholar
  7. JoubertWA, BritzTJ (1987) Isolation of saccharolytic dissimilatory sulfate-reducing bacteria. FEMS Microbiol Lett 48:35–40CrossRefGoogle Scholar
  8. KremerDR, HansenTA (1987) Glycerol and dihydroxyacetone dissimilation in Desulfovibrio strains. Arch Microbiol 147:249–256CrossRefGoogle Scholar
  9. KremerDR, Nienhuis-KuiperHE, HansenTA (1988) Ethanol dissimilation in Desulfovibrio. Arch Microbiol 150: 552–557CrossRefGoogle Scholar
  10. LeeJP, PeckHD (1971) Purification of the enzyme reducing bisulfite to trithionate from Desulfovibrio gigas and its identification as desulfoviridin. Biochem Biophys Res Commun 45:583–589CrossRefGoogle Scholar
  11. LowryOH, RosebroughNJ, FarrAL, RandallRJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275Google Scholar
  12. MacyJM, SnellenJE, HungateRE (1972) Use of syringe methods for anaerobiosis. Am J Clin Nutr 25:1318–1323CrossRefGoogle Scholar
  13. MarmurJ (1961) A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:2317–2324Google Scholar
  14. NanningaHJ, GottschalJC (1986) Isolation of a sulfate-reducing bacterium growing with methanol. FEMS Microbiol Ecol 38:125–130CrossRefGoogle Scholar
  15. NanningaHJ, GottschalJC (1987) Properties of Desulfovibrio carbinolicus sp. nov. and other sulfate-reducing bacteria isolated from an anaerobic-purification plant. Appl Environ Microbiol 51:572–579Google Scholar
  16. OllivierB, Cord-RuwischR, HatchikianEC, GarciaJL (1988) Characterization of Desulfovibrio fructosovorans sp. nov. Arch Microbiol 149:447–450CrossRefGoogle Scholar
  17. PfennigN (1978) Rhodocyclus purpureus gen. nov. and sp. nov., a ring-shaped, vitamin B12-requiring member of the family Rhodospirillaceae. Int J Syst Bacteriol 23:283–288CrossRefGoogle Scholar
  18. PfennigN, WiddelF, TrüperHG (1981) The dissimilatory sulfatereducing bacteria. In: StarrMP, StolpH, TrüperHG, BalowsA, SchlegelHG (eds) The prokaryotes. Springer, Berlin Heidelberg New York, pp 926–940CrossRefGoogle Scholar
  19. PostgateJR (1956) Cytochrom c 3 and desulphoviridin pigments of the anaerobic Desulphovibrio desulphuricans. J Gen Microbiol 14:545–572CrossRefGoogle Scholar
  20. PostgateJR (1959) A diagnostic reaction of Desulphovibrio desulphuricans. Nature 183:481–482CrossRefGoogle Scholar
  21. PostgateJR (1984) The sulfate reducing bacteria, 2nd edn. Cambridge University Press, LondonGoogle Scholar
  22. Qatibi AI (1990) Fermentation du lactate, du glycérol et des diols par les bactéries sulfato-réductrices du genre Desulfovibrio. Thèse Doctorat. Université Aix-Marseille I FranceGoogle Scholar
  23. QatibiAI, CayolJL, GarciaJL (1989) Glycerol degradation by Desulfovibrio sp., in pure culture and in coculture with Methanospirillum hungatei. In: BelaichJP, BruschiM, GarciaJL (eds) FEMS Symposium: Microbiology and biochemistry of strict anaerobes involved in interspecies hydrogen transfer. Plenum Press, New York, pp 447–449Google Scholar
  24. QatibiAI, GarciaJL (1989) 1,2- and 1,3-propanediol degradation by Desulfovibrio alcoholovorans sp., nov., in pure culture or through interspecies transfer. In: BelaichJP, BruschiM, GarciaJL (eds) FEMS Symposium: Microbiology and biochemistry of strict anaerobes involved in interspecies hydrogen transfer. Plenum Press, New York, pp 451–453Google Scholar
  25. Qatibi AI, Bories A, Garcia JL (1990) Sulfate reduction and anaerobic glycerol degradation by a mixed microbial culture. Curr Microbiol 22 (in press)CrossRefGoogle Scholar
  26. WiddelF (1988) Microbiology and ecology of sulfate- and sulfurreducing bacteria. In: ZehnderAJB (ed) Biology of anaerobic microorganisms. John Wiley, New York, pp 469–585Google Scholar
  27. WiddelF, PfennigN (1981) Studies on dissimilatory sulfate-reducting bacteria that decompose fatty acids. I. Isolation of new sulfate-reducing bacteria enriched with acetate from saline environments. Description of Desulfobacter postagatei gen. nov., sp. nov. Arch Microbiol 129:395–400CrossRefGoogle Scholar
  28. WiddelF, PfennigN (1984) Dissimilatory sulfate- or sulfur-reducing bacteria. In: KriegNR, HoltJG (eds) Bergey's manual of systematic bacteriology, 9th edn. Williams and Wilkins, Baltimore, pp 663–679Google Scholar

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • A. I. Qatibi
    • 1
  • V. Nivière
    • 2
  • J. L. Garcia
    • 1
  1. 1.ORSTOM, Laboratoire de MicrobiologieUniversité de ProvenceMarseille Cédex 3France
  2. 2.Laboratoire de Chimie BactérienneCNRSMarseille Cédex 9France

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