Archives of Microbiology

, Volume 155, Issue 2, pp 114–119 | Cite as

Cytochrome d expression and regulation pattern in free-living Rhizobium phaseoli

  • Blanca Barquera
  • Arturo Garcia-Horsman
  • Jose E. Escamilla
Original Papers


The respiratory system of Rhizobium phaseoli CFN42 in free-living cultures was studied. Cytochromes b, c, o and aa3 were found in fast growing cells cultured under forced aeration. Stationary aerobic cells, and semianaerobically grown cells showed decreased levels of cytochromes c, aa3 and o, concomitant with a significant increase of b type cytochromes and the synthesis of a new cytochrome, tentatively identified as cytochrome d. Cell membranes with the highest content of cytochrome d (semianaerobically grown cells) showed the highest respiratory activities with NADH, succinate, malate or ascorbate-TMPD (N,N,N′,N′-tetramethyl p-phenylendiamine). In the presence of either of the above electron donors, cytochrome d was clearly reduced. NADH dependent respiration in membranes of fast growing cells (no cytochrome d detected) was abolished by 25 μM KCN. This inhibitor concentration caused only 15–20% inhibition in membranes of semianaerobically grown cells (cyt d present). Moreover, in the presence of 1–5 mM KCN, the oxidation of cyt d and a b type cytochromes was spectrally detected. It is suggested that cyt d is a functional cytochrome in the respiratory system of free-living Rhizobia, probably acting as terminal oxidase.

Key words

Cytochrome d in Rhizobium phaseoli 


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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Blanca Barquera
    • 1
  • Arturo Garcia-Horsman
    • 1
  • Jose E. Escamilla
    • 1
  1. 1.Departamento de Microbiología, Instituto de Fisiología CelularUniversidad Nacional Autónoma de MéxicoMexico

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