Ferredoxin from the strict rumen anaerobe Selenomonas ruminantium has been purified to homogeneity and characterized with respect to its molecular weight and amino acid composition. The molecular weight of ferredoxin was 9,880. The A380/A280 absorbance ratio of the pure ferredoxin was 0.54 with a molar extinction coefficient of 31,000 M-1 cm-1 at 380 nm. Ferredoxin was reduced by cell-free extracts in the presence of hydrogen gas or pyruvate and acetyl coenzyme A.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
denotes an iron sulfur cluster containing x iron and y sulfur atoms
Chen J-S, Blanchard DK (1979) A simple hydrogenase-linked assay for ferredoxin and flavodoxin. Anal Biochem 93:216–222
Elliot JI, Brewer JM (1978) The inactivation of yeast enolase by 2,3-butadione. Arch Biochem Biophys 190:351–357
Hatchikian EC, Fardeau ML, Bruschi M, Belaich JP, Chapman A, Cammack R (1989) Isolation, characterization, and biological activity of the Methanococcus thermolithotrophicus ferredoxin. J Bacteriol 171:2384–2390
Hirs CHW (1967) Performic acid oxidation. Methods Enzymol 11:197–199
Hong J-S, Rabinowitz JC (1967) Preparation and properties of clostridial apoferredoxins. Biochem Biophys Res Commun 29:246–252
Matsubara H, Sasaki RM (1969) High recovery of tryptophan from acid hydrolysates of proteins. Biochem Biophys Res Commun 35:175–181
Melville SB, Michel TA, Macy JM (1987) Involvement of d-lactase and lactic acid racemase in the metabolism of glucose by Selenomonas ruminantium. FEMS Microbiol Lett 40:289–293
Melville SB, Michel TA, Macy JM (1988a) Pathway and sites for energy conservation in the metabolism of glucose by Selenomonas ruminantium. J Bacteriol 170:5298–5304
Melville SB, Michel TA, Macy JM (1988b) Regulation of carbon flow in Selenomonas ruminantium grown in glucose-limited continuous culture. J Bacteriol 170:5305–5311
Michel TA, Macy JM (1989) Purification of an enzyme responsible for acetate formation from acetyl coenzyme A in Selenomonas ruminantium. FEMS Microbiol Lett (in press)
Ragsdale SW, Ljungdahl LG (1984) Characterization of ferredoxin, and rubredoxin from Clostridium formicoaceticum grown in media with high and low iron contents. J Bacteriol 157:1–6
Saeki K, Wakabayashi S, Zumft WG, Matsubara H (1988) Pseudomonas stutzeri ferredoxin: Close similarity to Azotobacter vinelandii and Pseudomonas ovalis ferredoxins. J Biochem 104:242–246
Swank RT, Munkres KD (1971) Molecular weight analysis of oligopeptides by electrophoresis in polyacrylamide gel with sodium dodecyl sulfate. Anal Biochem 39:462–477
About this article
Cite this article
Michel, T.A., Macy, J.M. Ferredoxin from Selenomonas ruminantium . Arch. Microbiol. 153, 518–520 (1990). https://doi.org/10.1007/BF00248437
- Selenomonas ruminantium
- Anaerobic metabolism
- Pyruvate oxidation
- Pyruvate: ferredoxin oxidoreductase