Three proteins (components 1, 2, and 4) of the non-prolamin, 70% ethanol soluble fraction from the endosperm of Hordeum chilense have been identified as putative subunits of the tetrameric inhibitors active against insect α-amylases. In experiments carried out with the synthetic alloploid Tritordeum (H. chilense x Triticum turgidum conv. durum), previously described proteins from T. turgidum, designated CM2, CM3 and CM 16, have been also identified as subunits of α-amylase inhibitors. Genes for components 1 and 4 of H. chilense have been located in chromosomes 4Hch and 7Hch, based on the analysis of H. chilense-T.turgidum addition lines. Subunits of the inhibitors from wheat and from cultivated barley had been previously assigned to chromosomes of the same homoeology groups.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Aragoncillo C, Rodriguez-Loperena MA, Carbonero P, García-Olmedo F (1975) Chromosomal control of non-gliadin proteins from the ethanol extract of wheat endosperm. Theor Appl Genet 45:322–326
Barber D, Sanchez-Monge R, Mendez E, Lazaro A, García-Olmedo F, Salcedo G (1986a) New α-amylase and trypsin inhibitors among the CM proteins of barley (Hordeum vulgare L.). Biochim Biophys Acta 869:115–118
Barber D, Sanchez-Monge R, García-Olmedo F, Salcedo G, Mendez E (1986b) Evolutionary implications of sequential homologies among members of the trypsin/α-amylase inhibitor family (CM proteins) in wheat and barley. Biochim Biophys Acta 873:147–151
Buonocore V, De Biasi M-G, Giardina P, Poerio E, Silano V (1985) Purification and properties of an α-amylase tetrameric inhibitor from wheat kernel. Biochim Biophys Acta 831:40–48
Fernandez JA (1986) Caracteristicas geneticas y citogeneticas de Hordeum chilense y de su anfiploide x Triticum turgidum conv. durum. PhD Thesis, University of Alcala de Henares, Alcala de Henares, Madrid
Fernandez JA, Jouve N (1984) Giemsa C-banding of the chromosomes of Hordeum chilense and its amphiploid x Triticum turgidum conv. durum. Z Pflanzenzücht 93:212–221
Fernandez JA, Jouve N (1987a) Chromosomal location of structural genes controlling isozymes in Hordeum chilense. 1. 6-Phosfogluconate dehydrogenase and malate dehydrogenase. Theor Appl Genet 73:433–439
Fernandez JA, Jouve N (1987b) Chromosomal location of structural genes controlling isozymes in Hordeum chilense. 3. Esterases, glutamate oxaloacetate transaminase and phosphoglucomutase. Theor Appl Genet 73:690–698
Fernandez JA, Jouve N (1987c) The addition of Hordeum chilense chromosomes to Triticum turgidum conv. durum. Biochemical, karyological and morphological characterization. Euphytica (in press)
Fra-Mon P, Salcedo G, Aragoncillo C, García-Olmedo F (1984) Chromosomal assignment of genes encoding salt-soluble proteins (albumins and globulins) in wheat and related species. Theor Appl Genet 69:167–172
García-Olmedo F, Carbonero P, Jones BL (1982) Chromosomal locations of genes that control wheat endosperm proteins. In: Pomeranz Y (ed) Advances in cereal science and technology, vol V. AACC, St Paul Minn, pp 1–47
Love A (1982) Generic evolution of the wheatgrasses. Biol Zentralbl 101:199–212
Martin A, Sanchez-Monge Laguna E (1982) Cytology and morphology of the amphiploid Hordeum chilense x Triticum turgidum conv. durum. Euphytica 31:261–267
Miller TE, Reader SM, Ainsworth CC (1985) A chromosome of Hordeum chilense homoeologous to group 7 of wheat. Can J Genet Cytol 27:101–104
Molina-Cano JL, Fra-Mon P, Salcedo G, Aragoncillo C, Roca de Togores F, García-Olmedo F (1987) Morocco as a possible domestiation center for barley: biochemical and agromorphological evidence. Theor Apl Genet 73:531–536
Padilla JA, Martin A (1983) New hybrids between Hordeum chilense and tetraploid wheats. Cereal Res Commun 11:5–7
Rodriguez-Loperena MA, Aragoncillo C, Carbonero P, García-Olmedo F (1975) Heterogeneity of wheat endosperm proteolipids (CM proteins). Phytochemistry 14:1219–1223
Salcedo G, Sanchez-Monge R, Argamenteria A, Aragoncillo C (1980) The A-hordeins as a group of salt soluble hydrophobic proteins. Plant Sci Lett 19:109–119
Salcedo G, Sanchez-Monge R, Aragoncillo C (1982) The isolation and characterization of low molecular weight hydrophobic salt-soluble proteins from barley. J Exp Bot 33:1325–1331
Salcedo G, Fra-Mon P, Molina-Cano JL, Aragoncillo C, García-Olmedo F (1984) Genetics of CM-proteins (A-hordeins) in barley. Theor Appl Genet 68:53–59
Sanchez-Monge R, Barber D, Mendez E, García-Olmedo F, Salcedo G (1986a) Genes encoding α-amylase inhibitors are located in the short arms of chromosomes 3B, 3D and 6D of wheat (Triticum aestivum L.). Theor Appl Genet 72:108–113
Sanchez-Monge R, Gomez L, García-Olmedo F, Salcedo G (1986b) A tetrameric inhibitor of insect α-amylase from barley. FEBS Lett 207:105–109
Shewry PR, Miflin BJ (1985) Seed storage proteins of economically important cereals. In: Pomeranz Y (ed) Advances in cereal science and technology, vol III. AACC, St Paul Minn, pp 1–83
Communicated by G. Wenzel
About this article
Cite this article
Sanchez-Monge, R., Fernandez, J.A. & Salcedo, G. Subunits of tetrameric α-amylase inhibitors of Hordeum chilense are encoded by genes located in chromosomes 4Hch and 7Hch . Theoret. Appl. Genetics 74, 811–816 (1987). https://doi.org/10.1007/BF00247561
- Hordeum chilense
- Chromosomal location
- α-Amylase inhibitors