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Über den Einfluß von Phlorrhizin und Aminonucleosid auf den Phosphateinbau in Leber- und Nierenphosphatide

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1. Es werden die spezifischen Phosphat-Aktivitäten der Leber- und Nieren-Phosphatide der Ratte mit Hilfe radioaktiven Phosphats in vivo und in vitro untersucht.

2. Sie steigen bei Phosphatgabe in vivo unter Phlorrhizin in hoher Dosierung (750 mg/kg subcutan innerhalb 6 Std) um ein Viertel an; Unterschiede einzelner Phosphatidkomponenten lassen sich nicht erkennen. Sie bleiben unter Aminonucleosid (Nephrosestadium mit 122,5 mg/kg über 7 Tage) unverändert; nur die Phosphatidyläthanolamin-Komponente fällt in ihrer spezifischen Aktivität ab.

3. Bei Phosphatgabe in vitro zu Gewebshomogenaten vorbehandelter Tiere steigen die spezifischen Aktivitäten unter Phlorrhizin noch etwas stärker an. Ebenso wird ein derartiger Anstieg für Nierenhomogenate unter Aminonucleosid-Vorbehandlung sichtbar.

4. Bei Phosphatgabe in vitro zu Gewebshomogenaten unbehandelter Tiere läßt Phlorrhizinzugabe in vitro die spezifische Aktivität in Leberphosphatiden absinken, in Nierenphosphatiden stark ansteigen. Aminonucleosidzugabe erhöht sie nur in Nierenphosphatiden.


1. The specific activities of phosphate in rat liver and kidney phospholipides using radioactive phosphate was investigated in vivo and in vitro.

2. High doses of phlorrhizin (750 mg/kg subc. within 6 hours) increase the in vivo incorporation of phosphate about 25 per cent; differences within the single components could not be seen. Application of aminonucleoside (manifest nephrosis by 122,5 mg/kg for 7 days) did not change the incorporation; only a decrease of the phosphatidyl ethanolamine component was observed.

3. Application of phosphate to tissue homogenates of pretreated rats resulted in a more enhanced specific phosphate activity by phlorrhizin. A pretreatment with aminonucleoside produced a similar increase in kidney homogenates.

4. The phosphate specific activity diminishes after addition of both phosphate and phlorrhizin to liver homogenates in vitro; it increases strongly when given to kidney homogenates. Aminonucleoside raised only the phosphate incorporation in kidney homogenates in vitro.

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Herrn Professor Dr. O. Krayer zum 65. Geburtstag gewidmet.

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von Bruchhausen, F. Über den Einfluß von Phlorrhizin und Aminonucleosid auf den Phosphateinbau in Leber- und Nierenphosphatide. Naunyn - Schmiedebergs Arch 249, 21–34 (1964).

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