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Archives of Microbiology

, Volume 156, Issue 6, pp 497–500 | Cite as

Cytochromes and hydrogen-oxidizing activity in the thermophilic hydrogen-oxidizing bacteria related to the genus Hydrogenobacter

  • Trello Beffa
  • Margaret Berczy
  • Michel Aragno
Original Papers
  • 26 Downloads

Abstract

The highly thermophilic, hydrogen-oxidizing aerobic bacteria related to Hydrogenobacter possess a respiratory chain comprising a quinone and b-type (alpha band at 556 nm and 562 nm) and c-type (alpha band at 552 nm) cytochromes. They have no aa3-type cytochromes and their terminal oxidase is an o-type cytochrome. A polarographic method with an oxygen electrode was used for the measurement of the hydrogen-oxidizing activity. This activity was strongly inhibited by HQNO (2-N-heptyl-4-hydroxyquinoline N-oxide), an inhibitor of the respiratory chain in the quinone-cytochrome b region, and by KCN, an inhibitor of the terminal cytochrome oxidase. This study shows that the electrons released from hydrogen oxidation by the membrane-bound hydrogenase probably enter the respiratory chain at the level of the quinone-cytochrome b region.

Key words

Cytochromes b-c-o-types Hydrogen oxidation Hydrogenase Respiratory chain inhibitors Hydrogenobacter thermophilus Calderobacterium hydrogenophilum Hydrogenobacter sp. 

Abbreviations

HQNO

2-N-heptyl-4-hydroxyquinoline N-oxide

TMPD

N,N,N',N'-tetramethyl-p-phenylenediamine

DW

dry weight

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Trello Beffa
    • 1
  • Margaret Berczy
    • 1
  • Michel Aragno
    • 1
  1. 1.Laboratoire de Microbiologie, Institut de BotaniqueUniversité de NeuchâtelNeuchâtelSwitzerland

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