Archives of Microbiology

, Volume 156, Issue 6, pp 491–496 | Cite as

ATP synthesis from 2,3-diphosphoglycerate by cell-free extract of Methanobacterium thermoautotrophicum (strain ΔH)

  • Gert-Jan W. M. van Alebeek
  • Corné Klaassen
  • Jan T. Keltjens
  • Chris van der Drift
  • Godfried D. Vogels
Original Papers


Cell-free extracts of Methanobacterium thermoautotrophicum were found to catalyze ATP synthesis from an endogeneous substrate. Synthesis was stimulated under hydrogen atmosphere and inhibited by KCL (Ki=150 mM). Comparison of the properties of a number of cell constituents showed the endogeneous substrate to be 2,3-diphosphoglycerate. The compound is converted into 3-phosphoglycerate, and via 2-phosphoglycerate and phosphoenolpyruvate into pyruvate, at which the latter reaction is linked with ATP synthesis.

Key words

Methanogenic bacteria Methanobacterium thermoautotrophicum ATP synthesis 2,3-Diphosphoglycerate 2,3-Diphosphoglycerate phosphatase Cyclic 2,3-diphosphoglycerate 



Coenzyme M, 2-mercaptoethanesulfonate


methylcoenzyme m, 2-(methylthio)ethanesulfonate


7-mercaptoheptanoyl-l-threonine phosphate


the heterodisulfide of HS-CoM and HS-HTP


bolled cell-free extract




N-2-hydroxyethylpiperazine-N′-ethanesulfonic acid






cyclic 2,3-diphosphoglycerate






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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Gert-Jan W. M. van Alebeek
    • 1
  • Corné Klaassen
    • 1
  • Jan T. Keltjens
    • 1
  • Chris van der Drift
    • 1
  • Godfried D. Vogels
    • 1
  1. 1.Department of Microbiology, Faculty of ScienceUniversity of NijmegenNijmegenThe Netherlands

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