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Degradation of endogenous fructose during catabolism of sucrose and mannitol in halophilic archaebacteria

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Abstract

Metabolism of fructose arising endogenously from sucrose or mannitol was studied in halophilic archaebacteria Haloarcula vallismortis and Haloferax mediterranei. Activities of the enzymes of Embden-Meyerhof-Parnas (EMP) pathway, Entner-Doudoroff (ED) pathway and Pentose Phosphate (PP) pathway were examined in extracts of cells grown on sucrose or mannitol and compared to those grown on fructose and glucose. Sucrase and NAD-specific mannitol dehydrogenase were induced only when sucrose or mannitol respectively were the growth substrates. Endogenously arising fructose was metabolised in a manner similar to that for exogenously supplied fructose i.e. a modified EMP pathway initiated by ketohexokinase. While the enzymes for modified EMP pathway viz. ketohexokinase, 1-phosphofructokinase and fructose 1,6-bisphosphate aldolase were present under all growth conditions, their levels were elevated in presence of fructose. Besides, though fructose 1,6-bisphosphatase, phosphohexoseisomerase and glucose 6-phosphate dehydrogenase were present, the absence of 6-phosphogluconate dehydratase precluded routing of fructose through ED pathway, or through PP pathway directly as 6-phosphogluconate dehydrogenase was lacking. Fructose 1,6-bisphosphatase plays the unusual role of a catabolic enzyme in supporting the non-oxidative part of PP pathway. However the presence of constitutive levels of glucose dehydrogenase and 2-keto 3-deoxy 6-phosphogluconate aldolase when glucose or sucrose were growth substrates suggested that glucose breakdown took place via the modified ED pathway.

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Abbreviations

EMP:

Embden Meyerhof Parnas

ED:

Entner Doudoroff

PP:

pentose phosphate

KHK:

ketohexokinase

1-PFK:

1-phosphofructokinase

PEP-PTS:

phosphoenolpyruvate phosphotransferase

6-PFK:

6-phosphofructokinase

FBPase:

fructose 1,6-bisphosphatase

PHI:

phosphohexoseisomerase

G6P-DH:

glucose 6-phosphate dehydrogenase

6PG-DH:

6-phosphogluconate dehydrogenase

GAPDH:

glyceraldehyde 3-phosphate dehydrogenase

FIP:

fructose 1-phosphate

GSH:

reduced glutathione

2-ME:

β-mercaptoethanol

FBP:

fructose 1,6-bisphosphate

KDPG:

2-keto 3-deoxy 6-phosphogluconate

F6P:

fructose 6-phosphatez

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Correspondence to Wijaya Altekar.

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Altekar, W., Rangaswamy, V. Degradation of endogenous fructose during catabolism of sucrose and mannitol in halophilic archaebacteria. Arch. Microbiol. 158, 356–363 (1992). https://doi.org/10.1007/BF00245365

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Key words

  • Fructose metabolism (exogenous, endogenous)
  • Halophilic archaebacteria
  • EMP pathway (modified)