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Archives of Microbiology

, Volume 157, Issue 2, pp 131–134 | Cite as

Structure of the sugar-phosphate moiety of lipid A from lipooligosaccharide of Neisseria meningitidis group B, strain BC5S No. 125

Hydrolytic stability of phosphate and pyrophosphate substituents
  • Vyacheslav L. L'vov
  • Irina K. Verner
  • Larisa Yu. Musina
  • Alexander V. Rodionov
  • Anatoly V. Ignatenko
  • Alexander S. Shashkov
Original Papers
  • 27 Downloads

Abstract

On the basis of chemical and NMR data the partial structure of lipid A from lipooligosaccharide (LOS) of Neisseria meningitidis group B, strain BC5S No 125 was established. Lipid A consisted of disaccharide 2-deoxy-6-O-[2-deoxy-2-(3-hydroxytetradecanoylamino)-β-gluco-pyranosyl]-2-(3-hydroxytetradecanoylamino)-α-glucopyranose carrying the β-(2-aminoethyl)pyrophosphate residue at 0–4′ and the pyrophosphate or phosphate residue at 0–1. On hydrolysis of the acidic form of LOS with 1% acetic acid the substituent at 0–1 was practically completely removed whereas that at 0–4′ was stable. The analogous hydrolysis of the Mg-salt of LOS was accompanied by splitting off the pyrophosphate linkage in the substituent at 0–4′. Hydrolysis of LOS at pH 4.5 in the presence of SDS led mainly to a lipid A preparation retaining both pyrophosphate residues.

Key words

Neisseria meningitidis Lipooligosaccharide Lipid A Polar substituents Hydrolysis Partial structure 

Abbreviations

KDO

2-keto-3-deoxyoctulosonic acid

LA-I, LA-II

preparations of lipid A

LOS

lipooligosaccharide

LOS-H+

the acidic form of LOS

OS

oligosaccharide

TLC

thin-layer chromatography

GLC-MS

gas-liquid chromatography/mass spectrometry

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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Vyacheslav L. L'vov
    • 1
  • Irina K. Verner
    • 1
  • Larisa Yu. Musina
    • 2
  • Alexander V. Rodionov
    • 3
  • Anatoly V. Ignatenko
    • 4
  • Alexander S. Shashkov
    • 4
  1. 1.Institute of ImmunologyMinistry of Health of the USSRMoscowUSSR
  2. 2.M. M. Schemyakin Institute of Bioorganic ChemistryAcademy of Science of the USSRMoscowUSSR
  3. 3.N. F. Gamaleya Institute of Epidemiology and MicrobiologyAcademy of Medical Sciences of the USSRMoscowUSSR
  4. 4.N. D. Zelinsky Institute of Organic ChemistryAcademy of Sciences of the USSRMoscowUSSR

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