Archives of Microbiology

, Volume 157, Issue 2, pp 97–103 | Cite as

Clostridium quinii sp. nov., a new saccharolytic anaerobic bacterium isolated from granular sludge

  • Bo H. Svensson
  • Henri-Charles Dubourguier
  • Gerard Prensier
  • Alexander J. B. Zehnder
Original Papers


A new species of sporulating saccharolytic anaerobe, designated as Clostridium quinii sp. nov., is described. A gram-positive strain BS1, was isolated from the granular metanogenic sludge (UASB) from a waste-water treatment plant at a sugar refinery. The strain exhibits a series of morphological stages, developing from a spore to a small rod to a motile rod (peritrichous flagella) in the exponential growth phase, and then swelling to form cigar-shaped cells, exhibiting tumbling movements, in the late exponential growth phase before finally becoming large nonmotile ovoid cells in the stationary phase. Swelling occurs as a result of glucose being taken up and stored as a glycogen-like substance. The main fermentation products when growing on glucose is H2, CO2, formate, acetate and ethanol as well as small amounts of butyrate during exponential growth. Lactate is formed during the stationary phase, when glucose is abundant. Optimal conditions for growth is 40–45°C and pH of around 7.4. The type strain BS1 contains 28.0% mol G+C.

Key words

Pleomorphic Clostridium sp. nov. Saccharolytic Glucose uptake Swelling cells Morphology 


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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Bo H. Svensson
    • 1
  • Henri-Charles Dubourguier
    • 2
  • Gerard Prensier
    • 3
  • Alexander J. B. Zehnder
    • 4
  1. 1.Department of MicrobiologySwedish University of Agricultural SciencesUppsalaSweden
  2. 2.Laboratoire de MicrobiologieU.S.T.L.Villeneuve d'Asq CedexFrance
  3. 3.Laboratoire de MicrobiologieUniversité de Clement-FdAubièreFrance
  4. 4.Department of MicrobiologyAgricultural UniversityWageningenThe Netherlands

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