Advertisement

Archives of Microbiology

, Volume 157, Issue 2, pp 97–103 | Cite as

Clostridium quinii sp. nov., a new saccharolytic anaerobic bacterium isolated from granular sludge

  • Bo H. Svensson
  • Henri-Charles Dubourguier
  • Gerard Prensier
  • Alexander J. B. Zehnder
Original Papers
  • 119 Downloads

Abstract

A new species of sporulating saccharolytic anaerobe, designated as Clostridium quinii sp. nov., is described. A gram-positive strain BS1, was isolated from the granular metanogenic sludge (UASB) from a waste-water treatment plant at a sugar refinery. The strain exhibits a series of morphological stages, developing from a spore to a small rod to a motile rod (peritrichous flagella) in the exponential growth phase, and then swelling to form cigar-shaped cells, exhibiting tumbling movements, in the late exponential growth phase before finally becoming large nonmotile ovoid cells in the stationary phase. Swelling occurs as a result of glucose being taken up and stored as a glycogen-like substance. The main fermentation products when growing on glucose is H2, CO2, formate, acetate and ethanol as well as small amounts of butyrate during exponential growth. Lactate is formed during the stationary phase, when glucose is abundant. Optimal conditions for growth is 40–45°C and pH of around 7.4. The type strain BS1 contains 28.0% mol G+C.

Key words

Pleomorphic Clostridium sp. nov. Saccharolytic Glucose uptake Swelling cells Morphology 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Adamse AD (1980) New isolation of Clostridium aceticum (Wieringa)1. Antonie van Leeuwenhoek 46: 523–531CrossRefGoogle Scholar
  2. Adamse AD, Dienema MN, Zehnder AJB (1984) Studies on bacterial activities in aerobic and anacrobic waste water purification. Antonie van Leeuwenhoek 50: 665–682CrossRefGoogle Scholar
  3. Brough BE, Reid TC, Howard BH (1970) The biochemistry of the rumen bacterium “Quin's Oval” — Part 1. Fermentation of carbohydrates. N Z J Sci 13: 570–575Google Scholar
  4. Buck JD (1982) Nonstaining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol 44: 992–993PubMedPubMedCentralGoogle Scholar
  5. Cato EP, George WL, Finegold SM (1986) Genus Clostridium. Prazmowski 1880, 23AL. In: Sneath PHA (ed) Bergey's manual of systematic bacteriology, vol. 2. William & Wilkins, Baltimore London, pp 1141–1200Google Scholar
  6. Chung KT (1976) Inhibitory effect of H2 on growth of Clostridium cellobioparum. Appl Environ Microbiol 31: 342–348PubMedPubMedCentralGoogle Scholar
  7. Dolfing J, Bloemen WGBM (1985) Activity measurements as a tool to characterize the microbial composition of methanogenic environments. J Microbiol Methods 4: 1–12CrossRefGoogle Scholar
  8. Ferragut C, Leclerc H (1976) Etude comparative des méthodes de détermination du Tm de l'ADN bactérien. Ann Microbiol (Inst Pasteur) 127: 223–235Google Scholar
  9. Herbert D, Phipps PJ, Strange RE (1971) Chemical analysis of microbial cells. In: Norris JR, Ribbons DW (eds) Methods in microbiology, vol 5B. Academic Press, London, pp 209–344Google Scholar
  10. Hoffman A, Dimroth P (1990) The ATPase of Bacillus alcalophilus. Purification and properties of the enzyme. Eur J Biochem 194: 423–490CrossRefGoogle Scholar
  11. Holdeman LV, Cato EP, Moore WEC (1977) Anaerobic laboratory manual, 4th edn. Virginia Polytechnical Institute and State University, Blacksburg, Va., USAGoogle Scholar
  12. Johnson JL (1981) Genetic characterization. In: Gerhardt P, Murry RGE, Costilow RN, Nester EW, Wood WA, Kodeg NR, Philips GB (eds) Manual of methods for general bacteriology. ASM, Washington DC, pp 450–472Google Scholar
  13. Jones DT, Westhuizen A van der, Long S, Allcock ER, Ried SJ, Woods DR (1982) Solvent production and morphological changes in Clostridium acetobutylicum. Appl Environ Microbiol 43: 1434–1439PubMedPubMedCentralGoogle Scholar
  14. Kelly WJ, Asamudson RV, Hopcroft DH (1987) Isolation and characterization of a strictly anaerobic cellulolytic spore former: Clostridium chartatabidium sp. nov. Arch Microbiol 147: 169–173CrossRefGoogle Scholar
  15. Kim BH, Bellows P, Datta R, Zeikus JG (1984) Control of carbon and electron flow in Clostridium acetobutylicum fermentations. Utilization of carbon monoxide to inhibit hydrogen production and to enhance butanol yields. Appl Environ Microbiol 53: 644–650Google Scholar
  16. LeRuyet P, Dubourgier H-C, Albagnac G, Prensier G (1985) Characterization of Clostridium thermolacticum sp. nov., a hydrolytic thermophilic anaerobe producing high amounts of lactate. Syst Appl Microbiol 6: 196–202CrossRefGoogle Scholar
  17. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275Google Scholar
  18. Orpin CG (1972) The culture in vitro of the rumen bacterium Quin's Oval. J Gen Microbiol 73: 523–530CrossRefGoogle Scholar
  19. Scholten-Koerselman I, Houward F, Janssen P, Zehnder AJB (1986) Bacteriodes xylanolyticus sp. nov., a xylanolytic bacterium from methane producing cattle manure. Antoine van Leeuwenhoek 52: 543–554CrossRefGoogle Scholar
  20. Stouthamer AH (1973) A theoretical study on the amount of ATP required for synthesis of microbial cell material. Antonie van Leeuwenhoek 39: 545–565CrossRefGoogle Scholar
  21. Thiery JP (1967) Mise en évidence des polysaccharides sur coupe fine en microscopie électronique. J Microsc 67: 987–1018Google Scholar
  22. Vicini JL, Brulla WJ, Davis CL, Bryant MP (1987) Quin's Oval and other microbiota in the rumens of molasses-fed sheep. Appl Environ Microbiol 53: 1273–1276PubMedPubMedCentralGoogle Scholar
  23. Wicken AJ, Howard BH (1967) On the taxonomic status of “Quin's Oval” organisms. J Gen Microbiol 47: 207–211CrossRefGoogle Scholar
  24. Wolin EA, Wolin MJ, Wolfe RS (1963) Formation of methane by bacterial extracts. J Biol Chem 238: 2882–2886Google Scholar
  25. Zehnder AJB, Huser BA, Brock TD, Wuhrman K (1980) Characterization of an acetate-decarboxylating, non-hydrogen-oxidizing methane bacterium. Arch Microbiol 124: 1–11CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Bo H. Svensson
    • 1
  • Henri-Charles Dubourguier
    • 2
  • Gerard Prensier
    • 3
  • Alexander J. B. Zehnder
    • 4
  1. 1.Department of MicrobiologySwedish University of Agricultural SciencesUppsalaSweden
  2. 2.Laboratoire de MicrobiologieU.S.T.L.Villeneuve d'Asq CedexFrance
  3. 3.Laboratoire de MicrobiologieUniversité de Clement-FdAubièreFrance
  4. 4.Department of MicrobiologyAgricultural UniversityWageningenThe Netherlands

Personalised recommendations