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Archives of Microbiology

, Volume 154, Issue 5, pp 518–520 | Cite as

Electrophoretic detection of multiple protein kinases in the entomopathogenic fungus Metarhizium anisopliae

  • Raymond J. St. Leger
  • Donald W. Roberts
  • Richard C. Staples
Short Communication

Abstract

Multiple membrane bound protein kinases were detected following electrophoresis of a membrane preparation from Metarhizium anisopliae on sodium dodecyl sulfate-polyacrylamide gels. After renaturation, polypeptides in the gel were incubated with [γ-32P]ATP for phosphorylation of the kinases themselves or of histones or casein included in the gel. Eight radioactive bands were detected in ungerminated conidia, a 40 kDa band predominated. Sixteen bands (Mr range 28–72 kDa) were detected in both a crude extract and membrane fraction from mycelia. All of the bands were intensified when casein was included in the gel; four bands including the major 40-kDa band were intensified with mixed histones. The same bands were detected when the phosphorylation was carried out with or without Ca2+, calmodulin, EGTA, cyclic AMP, spermine or heparin. Most of the phosphorylating enzymes fit the general descriptions of casein kinases.

Key words

Membrane-bound protein kinases Phosphorylation of casein/histones Metarhizium anisopliae 

Abbreviations

cAMP

Cyclic AMP

SDA

Sabouraud dextrose agar

SDB

Sabouraud dextrose broth

SDS

Sodium dodecyl sulfate

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Raymond J. St. Leger
    • 1
  • Donald W. Roberts
    • 1
  • Richard C. Staples
    • 1
  1. 1.Boyce Thompson Institute for Plant ResearchCornell UniversityIthacaUSA

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