Advertisement

Archives of Microbiology

, Volume 154, Issue 5, pp 514–517 | Cite as

Characterization of a mutant of the yeast Candida maltosa defective in catabolite inactivation of gluconeogenetic enzymes

  • K. H. Hofmann
  • E. Polnisch
Original Papers

Abstract

A spontaneous mutant of the yeast Candida maltosa SBUG 700 was isolated showing pseudohyphal marphology under all growth conditions tested. The C. maltosa PHM mutant takes up glucose with the kinetics of C. maltosa SBUG 700 and starved cells contain the same cyclic AMP concentration. Addition of glucose to the PHM mutant does not result in an increase of the intracellular cyclic AMP level and in catabolite inactivation of fructose-1,6-bisphosphatase, malate dehydrogenase and phosphoenolpyruvate carboxykinase. However, addition of 2,4-dinitrophenol is followed by a rapid, transient increase of the cyclic AMP level in the mutant cells, but not by catabolite inactivation. These results show that a common mechanism might be responsible for catabolite inactivation and glucose-induced cAMP signaling or that glucose-induced cAMP signaling is required for catabolite inactivation in C. maltosa.

Key words

Candida maltosa Mutant Pseudohyphal morphology Glucose uptake Cyclic AMP Catabolite inactivation 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Broek D, Toda T, Michaelis T, Levin L, Birchmeier C, Zoller M, Powers S, Wigler M (1989) The Saccharomyces cerevisiae CDC 25 gene product regulates the RAS/adenylate cyclase pathway. Cell 48: 789–799CrossRefGoogle Scholar
  2. Hanes CS (1932) Studies on plant amylases. I. The effect of starch concentrations upon the velocity of hydrolysis by the amylases of germinated barley. Biochem J 26: 1406–1421CrossRefGoogle Scholar
  3. Hansen RJ, Hinze H, Holzer H (1976) Assay of phosphoenolpyruvate-carboxykinase in crude yeast extracts. Anal Biochem 74: 576–584CrossRefGoogle Scholar
  4. Hofmann KH, Polnisch E (1990) Cyclic AMP-dependent phosphorylation of fructose-1,6-bisphosphatase and other proteins in the yeast Candida maltosa. J Basic Microbiol 30: (in press)CrossRefGoogle Scholar
  5. Hofmann KH, Schauer F (1988) Utilization of phenol by hydrocarbon assimilating yeasts. Antonie van Leeuwenhoek 54: 179–188CrossRefGoogle Scholar
  6. Hofmann KH, Vogt U (1987) Induction of phenol assimilation in chemostat cultures of Candida maltosa L4. J Basic Microbiol 27: 441–447CrossRefGoogle Scholar
  7. Hofmann KH, Vogt U (1988) Degradation of phenol by yeasts in the presence of n-hexadecane under growth conditions in a stirred reactor. Zentrabl Mikrobiol 143: 87–91CrossRefGoogle Scholar
  8. Larsen AD, Sypherd PS (1974) Cyclic adenosine 3′, 5′-monophosphate and morphogenesis in Mucor racemosus. J Bacteriol 117: 432–438PubMedPubMedCentralGoogle Scholar
  9. Levitzki A (1988) Transmembrane signalling to adenylate cyclase in mammalian cells and in Saccharomyces cerevisiae. Trends Biochem Sci 13: 298–301CrossRefGoogle Scholar
  10. Matsumoto K, Uno I, Ishikawa T (1985) Genetic analysis of the role of cAMP in yeast. Yeast 1: 15–24CrossRefGoogle Scholar
  11. Munder T, Küntzel H (1989) Glucose-induced cAMP signaling in Saccharomyces cerevisiae is mediated by the CDC 25 protein. FEBS Lett 242: 341–345CrossRefGoogle Scholar
  12. Niimi M, Niimi K, Tokunaga J, Nakayama H (1980) Changes in cyclic nucleotide levels and dimorphic transition in Candida albicans. J Bacteriol 142: 1010–1014PubMedPubMedCentralGoogle Scholar
  13. Omi K, Kamihara T (1989) Accumulation of cAMP in the cells of Candida tropicalis at an early stage of ethanol-induced filamentous growth and its prevention by myo-inositol. Biochem Biophys Res Commun 162: 646–650CrossRefGoogle Scholar
  14. Pall MI (1984) Is there a general paradigm of cyclic AMP action in eukaryotes? Mol Cell Biochem 58: 187–191CrossRefGoogle Scholar
  15. Polnisch E, Hofmann K (1989) Cyclic AMP, fructose-2,6-bisphosphate and catabolite inactivation of enzymes of the hydrocarbon assimilating yeast Candida maltosa. Arch Microbiol 152: 269–272CrossRefGoogle Scholar
  16. Postma E, Scheffers WA, VanDijken JP (1988) Adaptation of the kinetics of the glucose transport to environmental conditions in the yeast Candida utilis CBS 621: a continuous culture study. J Gen Microbiol 134: 1109–1116Google Scholar
  17. Postma E, Scheffers WA, VanDijken JP (1989) Kinetics of growth and glucose transport in glucose-limited chemostat cultures of Saccharomyces cerevisiae CBS 8066. Yeast 5: 159–165CrossRefGoogle Scholar
  18. Robinson LC, Gibbs JB, Marshall MS, Sigal IS, Tatchell K (1987) CDC 25: a component of the RAS-adenylate cyclase pathway in Saccharomyces cerevisiae. Science 235: 1218–1221CrossRefGoogle Scholar
  19. Thevelein JM, Beullens M, Mbonyi K, VanAeist L (1989) The glucose-induced CDC 25-and RAS-mediated cAMP signal in the yeast Saccharomyces cerevisiae. Yeast 5: 421–425Google Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • K. H. Hofmann
    • 1
  • E. Polnisch
    • 1
  1. 1.Sektion BiologieErnst-Moritz-Arndt-Universität GreifswaldGreifswaldGermany

Personalised recommendations