Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Protease-producing psychrotrophic bacteria isolated from Antarctica

  • 138 Accesses

  • 17 Citations

Abstract

The extracellular protease production capacity of 840 bacterial strains isolated during the austral summers of 1989/90 and 1991/92 from different sources of the Antarctic ecosystem was analysed in skim-milk agar plates. Thirty-four psychrotrophic strains were selected, classified at genus level and tested from proteolytic activity by the azocasein method from the cell-free supernatant of submerged cultures. Thirty-two of the selected strains were Gram-negative bacteria and Pseudomonas was the predominant genus. Three Pseudomonas maltophilia strains showed the highest levels of proteolytic activity at 20°C. No correlation was observed between the proteolytic activity estimated by the ring of hydrolysis in skim-milk agar plates and the activity measured by the azocasein method. The results suggest that these psychrotrophic strains are potentially useful for developing a biotechnological process to produce proteases with high activity at moderate temperatures.

This is a preview of subscription content, log in to check access.

References

  1. Amrute SB, Corpe WA (1978) Production of extracellular protease y Pseudomonas fluorescens W. In: Underkefler LA (ed) Developments in industrial microbiology. Plenum Press, New York, 19, pp 465–471

  2. Aunstrup K (1974) Industrial Production of Proteolytic Enzymes. In: Spencer B (ed) Industrial aspects of biochemistry. North Holland — American Elsevier, Amsterdam, pp 23–46

  3. Bahn M, Schmid RD (1987) Enzymes for detergents. In: Biotec, Vol 1, Gustav Fisher, Stuttgart, New York, pp 119–130

  4. Boethling RS (1975) Purification and properties of a serine protease from Pseudomonas maltophilia. J Bacteriol 121:933–941

  5. Bouvy M, Delille D (1987) Numerical taxonomy of bacterial communities associated with a sub-Antarctic mussel bed. Helgol Wiss Meeresunters 41:415–424

  6. Bouvy M, Delille D (1988) Spatial and temporal variations in Antarctic and sub-Antarctic bacterioplankton. Neth J Sea Res 20:291–303

  7. Charney J, Tomarelli RM (1947) A colorimetric method for the determination of the proteolytic activity of duodenal juice. J Biol Chem 171:501–507

  8. Delille D (1987) Spatial distribution of coastal Antarctic seawater bacteria: relationship with avifauna. Polar Biol 8:55–60

  9. Delille D (1990) Seasonal changes of sub-Antarctic heterotrophic bacterioplankton. Arch Hydrobiol 119:267–277

  10. Delille D (1992) Marine bacterioplankton at the Weddell Sea ice edge: distribution of psychrophilic and psychrotrophic populations. Polar Biol 12:205–210

  11. Delille D (1993) Seasonal changes in the abundance and composition of marine heterotrophic bacterial communities in an Antarctic coastal area. Polar Biol 12:205–210

  12. Delille D, Bouby M (1989) Bacterial responses to natural organic input in a marine subAntarctic area. Hydrobiologia 182:225–238

  13. Delille D, Perret E (1989) Influence of temperature on the growth potential of southern polar marine bacteria. Microb Ecol 18:117–123

  14. Delille D, Bouby M, Cahet G (1988) Short term variations of bacterioplankton in Antarctic zone: Terre Adelie area. Microb Ecol 15:293–309

  15. Duchene JC, Imbaud P, Delille D (1988) Associated bacterial microflora of a sub-Antarctic polychaete worm Thelepus setosus. Arch Hydrobiol 112:221–231

  16. Espeche ME, Molina MG, Fraile ER (1987) Enzymatic activities of psychrotrophic bacteria from Antarctic krill. In: El-Sayed SZ, Tomo AP (ededs) Antarctic aquatic biology. BIOMASS Sci Ser 7:133–139

  17. Fuhrman JA, Azam F (1980) Bacterioplankton secondary production estimates for coastal waters of British Columbia, Antarctica and California. Appl. Environ Microbiol 39:1085–1095

  18. Fujiwara N, Yamamoto K (1987) Production of alkaline protease in a low-cost medium by alkalophilic Bacillus sp. and properties of the enzyme. J Ferment Technol 651:345–348

  19. Juan SM, Cazzulo JJ (1976) Produccion de proteasa extracelular por una bacteria criofila de agua dulce. Rev Soc Argent Microbiol 8:8–13

  20. Kato N, Adachi S, Takeuchi K, Morihara K, Tani Y, Ogata K (1974) Substrate especificities of the proteases from a marine-psychrophilic bacterium, Pseudomonas sp. N∘. 548. Agric Biol Chem 38:103–109

  21. Keay L, Wildi BS (1970) Proteases of the genus Bacillus. I. Neutral proteases. Biotechnol Bioeng 12:179–212

  22. Keay L, Moser PW, Wildi BS (1970) Proteases of the genus Bacillus. II. Alkaline proteases. Biotechnol Bioeng 12:213–249

  23. Koike I, Rönner U, Holm-Hansen O (1981) Microbial nitrogen metabolism in the Scotia Sea. Antarct J US 16:165–166

  24. Koike I, Holm-Hansen O, Biggs DC (1986) Inorganic nutrient metabolism by Antarctic phytoplankton with special reference to ammonium cycling. Mar Ecol Prog Ser 30:105–116

  25. Kole MM, Draper I, Gerson DF (1988) Protease production by Bacillus subtilis in oxygen-controlled, glucose-fed batch fermentations. Appl Microbiol Biotechnol 28:404–408

  26. Law BA (1979) Reviews of the progress of dairy science: Enzymes of psychrotrophic bacteria and their effects on milk and milk products. J Dairy Res 46:573–588

  27. Lyman J, Fleming RH (1940) Composition of sea water. J Mar Res 3:134–146

  28. MacCormack WP, Fraile ER (1990) Bacterial flora of newly caught Antarctic fish Notothenia neglecta. Polar Biol 10:413–417

  29. McGuire AJ, Franzmann PD, McMeekin TA (1987) Flectobacillus glomeratus sp. nov., a curved, non-motile, pigmented bacterium isolated from Antarctic marine environments. Syst Appl Microbiol 9:265–272

  30. Molin G, Stenstrom I, Ternstrom A (1983) The microbial flora of herring fillets after storage in carbon dioxide, nitrogen or air at 2°C. J Appl Bacteriol 55:49–56

  31. Morita RY (1975) Psychrophilic bacteria. Bacteriol Rev 39: 144–167

  32. Shewan JM (1971) The microbiology of fish and fishery products. A progress report. J Appl Bacteriol 34:299–315

  33. Shewan JM, Hobbs G, Hodkiss W (1960a) A determinative scheme for the identification of certain genera of Gram-negative bacteria, with special reference to the Pseudomonadaceae. J Appl Bacteriol 23:379–390

  34. Shewan JM, Hobbs G, Hodkiss W (1960b) The Pseudomonas and Achromobacter groups of bacteria in the spoilage of marine white fish. J Appl Bacteriol 23:463–468

  35. Shivaji S, Shyamala Rao N, Saisree L, Sheth V, Reddy GSN, Bhargava PH (1989) Isolation and identification of Pseudomonas spp. from Schirmacher Oasis, Antarctica. Appl Environ Microbiol 55:767–770

  36. Tsuchida O, Yamagata Y, Ishizuka T, Arai T, Yamada JI, Takeuchi M, Ichishima E (1986) An alkaline proteinase from an alkalophilic Bacillus sp. Curr Microbiol 14:7–12

  37. Wynn-Williams DD (1983) Distribution and characteristics of Chromobacterium in the maritime and sub-Antarctic. Polar Biol 2:101–108

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Vazquez, S.C., Rios Merino, L.N., MacCormack, W.P. et al. Protease-producing psychrotrophic bacteria isolated from Antarctica. Polar Biol 15, 131–135 (1995). https://doi.org/10.1007/BF00241051

Download citation

Keywords

  • Hydrolysis
  • Pseudomonas
  • Proteolytic Activity
  • Production Capacity
  • Moderate Temperature