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Anion-dependent transport of thallous ions through human erythrocyte membrane

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Summary

Unidirectional fluxes of 204Tl+ through the human red blood cell membrane were measured. The inward rate coefficient measured in a K+-free saline was 15.6±0.6 hr−1. The influx of Tl+ could be partially inhibited with 0.1mm ouabain (by 28%), 0.1mm DIDS (by 50%) or 1mm furosemide (by 51%). The inhibitory effects of ouabain and DIDS or furosemide were additive. Half-maximal responses were seen at 0.72 μ m and 0.22mm concentrations of DIDS and furosemide, respectively. A similar action of these blockers on Tl+ influx was observed in the erythrocytes incubated in MgCl2-sucrose media. The outward rate coefficient of 204Tl was also inhibited by DIDS and furosemide (by 65 and 52%, respectively). Rate coefficients of 204Tl influx and efflux decreased significantly in the red cells exposed to Cl-free media (NaNO3 or Mg(NO3)2-sucrose). Under these conditions addition of DIDS and furosemide led to only a small inhibition of Tl+ fluxes. There was a linear increase in Tl+ influx with rising of external Cl concentration within 80–155mm or HCO 3 concentration from 20 to 40mm when the sum of anions was kept constant (155mm) with NO 3 . The HCO 3 -stimulated Tl+ influx was completely blocked by 0.05mm DIDS but only 67% by 1mm furosemide. The present study provides direct evidence for the occurrence of Cl (HCO 3 )-dependent, DIDS-sensitive movement of Tl+ across the human erythrocyte membrane in both directions. Under physiological conditions, about half of net Tl+ fluxes occurs due to an anion exchange mechanism. Our data fail to detect a contribution of the Na-K-Cl cotransport system to Tl+ transport in human erythrocytes.

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Skulskii, I.A., Gusev, G.P., Sherstobitov, A.O. et al. Anion-dependent transport of thallous ions through human erythrocyte membrane. J. Membarin Biol. 130, 219–225 (1992). https://doi.org/10.1007/BF00240479

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Key words

  • thallium transport
  • anion exchanger
  • human erythrocytes