The biodegradation curve of octadecylbis(2-hydroxyethyl)amine determined in a Closed Bottle test suggested an initial oxidation of the alkyl chain and a subsequent degradation of the diethanolamine formed. Using the sludge from the test as inoculum, a bacterium capable of utilizing octadecylbis(2-hydroxyethyl)amine as sole source of carbon and energy was isolated. This bacterium also utilized various other alkylbis(2-hydroxyethyl)amines and octadecylpolyoxyethylene(5)amide. Respirometric studies and the formation of diethanolamine by a washed cell suspension of the pure culture showed that the bacterium only oxidized the alkyl chain. Furthermore, in cell-free extracts a dehydrogenase activity catalysing the oxidation of octadecylbis(2-hydroxyethyl)amine was detected.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
BaleuxB & CaumetteP (1974) Biodegradation of nonionic surfactants. Study of a new experimental method and screening test. Rev. Inst. Pasteur Lyon 7: 278–297
BeinertH, GreenDE, HeleP, HiftH, KorfvonRW & RamakrishnanCW (1953). The acetate activating enzyme system of heart muscle. J. Biol. Chem. 203: 35–45
BerthP, GerikeP, GodeP & SteberJ (1984) Ecological evaluation of important surfactants. Surfactant Congress 8: 1227–236
ColbyJ & ZatmanLJ (1971) Trimethylamine metabolism in obligate and facultative methylotrophs. Biochem. J. 132: 101–112
CrippsRE & NobleAS (1973) The metabolism of nitriloacetate by a pseudomonad. Biochem. J. 136: 1059–1068
DixonGH & KornbergHL (1959) Assay methods for key enzymes of the glyoxylate cycle. Biochem. J. 3: 72
FischerWK (1972) Correlation between the constitution and biodegradability of nonionic surfactants. Surfactant Congress 6: 73–75
FischerWK (1981) Important aspects of ecological evaluation of fatty alcohols and their derivatives. In: Fettalkohol Rohstoffe Verfahren und Verwendung. Henkel, Dusseldorf, English Edition (pp 188–222)
LargePJ (1971) The oxidative cleavage of alkyl nitrogen bonds in microorganisms. Xenobiotica 1: 457–467
LargePJ, BoultonCA & CrabbeMJC (1972) The reduced nicotinamide-adenine dinucleotide phosphate- and oxygen-dependent N-oxygenation of trimethylamine by Pseudomonas aminovorans. Biochem. J. 128: 137–138
MeibergJBM & HarderW (1978) Aerobic and anaerobic metabolism of trimethylamine, dimethylamine and methylamine in Hyphomicrobium X. J. Gen. Microbiol. 106: 265–276
OECD (1982) Guidelines for testing of chemicals, section 3; Degradation and accumulation No. 301 D: ready biodegradability, Closed Bottle test. Paris, France
Ruiz CruzJ & Dobarganes GarciaMC (1978) Pollution of natural water by synthetic detergents XIII. Biodegradation of non-ionic surfactants in river water and determination of their biodegradability by different test methods. Grasas Aceites 29: 11–17
SchöberlP, BockKJ & HuberL (1988) Okologisch relevante Daten von Tensiden in Wasch und Reinigungsmitteln. Tenside Detergents 25: 86–98
SwisherRD (1987) Surfactant Biodegradation, 2nd edition. Surfactant Science Series, Vol. 18. Marcel Dekker Inc, New York and Basel
TiedjeJM, MasonBB, WarrenCB & MalecEJ (1973) Metabolism of nitrilotriacetate by cells of Pseudomonas species. Appl. Microbiol. 25: 811–825
UetzT, SchneiderR, SnozziM & EgliT (1992) Purification and characterization of a two-component monooxygenase that hydroxylates nitrilotriacetate from Chelatobacter strain ATCC 29600. J. Bacteriol. 174(4): 1179–1188
VerdouwH, EchteldCJAvan & DekkersEMJ (1978) Ammonia determination based on indophenol formation with sodium salicylate. Water Res. 12: 399–402
VisniacW & SanterM (1957) The thiobaccilli. Bacteriol. Rev. 21: 195–213
WatersJ & KupferW (1976) The determination of cationic surfactants in the presence of anionic surfactant in biodegradation test liquors. Anal. Chimica Acta 85: 241–251
WheelerPR, BulmerK & RadledgeC (1991) Fatty acid oxidation and β-oxidation complex in Mycobacterium leprae and two axenically mycobacteria that are pathogens. J. Gen. Microbiol. 137: 885–893
WilliamsGR & CallelyAG (1982) The biodegradation of diethanolamine and triethanolamine in a yellow Gram-negative rod. J. Gen. Microbiol. 128: 1203–1209
About this article
Cite this article
van Ginkel, C.G., Kroon, A.G.M. Metabolic pathway for the biodegradation of octadecylbis(2-hydroxyethyl)amine. Biodegradation 3, 435–443 (1992). https://doi.org/10.1007/BF00240365
- non-ionic surfactant
- central fission