A re-examination of goldfish liver was made through the use of SEM of fractured samples and TEM of ultrathin-sections and freeze-etch replicas. Several new hepatic fine structures described in the present study are morphologically similar to those reported previously in many higher vertebrates including mammals. Hepatic sinusoids of goldfish contain fenestrations which are arranged into sieve plates. Although the hepatic plates are made up of two layers of hepatocytes, the parenchymal cells of goldfish liver are morphologically similar to mammalian hepatocytes, particularly with respect to the sinusoidal surfaces which are studded with numerous microvilli. The intercellular surfaces of hepatocytes have both nexus and desmosomal junctions, similar to those found in various epithelial cells of higher vertebrates, as cell attachments and communication foci. Tight junctions are found mainly between the openings of the intracellular bile canaliculi and the intralobular bile ductules which are situated in the center of the bicellular hepatic plate.
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Compagno, J., Grisham, J.W.: Scanning electron microscopy of extrahepatic biliary obstruction. Arch. Path. 97, 348–351 (1974)
Elias, H., Bengelsdorf, H.: The structure of the liver of vertebrates. Acta Anat. (Basel) 15, 297–337 (1952)
Grisham, J.W., Nopanitaya, W., Compagno, J., Nagel, A.E.H.: Scanning electron microscopy of normal rat liver: The surface structure of its cells and tissue components. Am. J. Anat. 144, 295–322 (1975)
Haar, J.L., Hightower, J.A.: A light and electron microscopic investigation of the hepatic parenchyma of the adult newt, Notophthalmus viridescens. Anat. Rec. 185, 313–324 (1976)
Ito, T.: Recent advances in the study on the fine structure of the hepatic sinusoidal wall; a review. Gunna Rep. Med. Sci. 6, 119–163 (1973)
Itoshima, T., Kobayashi, T., Shimada, Y., Murakami, T.: Fenestrated endothelium of the liver sinusoids of the guinea pig as revealed by scanning electron microscopy. Arch. Histol. Jpn. 37, 15–24 (1974)
Moor, H.: Use of freeze-etching in the study of biological ultrastructure. Int. Rev. Exp. Pathol. 5, 179–216 (1966)
Motta, P.: A scanning electron microscopy study of the rat liver sinusoid endothelial and Kupffer cells. Cell Tissue Res. 164, 371–385 (1975)
Motta, P., Fumagalli, G.: Structure of rat bile canaliculi as revealed by scanning electron microscopy. Anat. Rec. 182, 499–531 (1975)
Motta, P., Porter, K.R.: Structure of rat liver sinusoids and associated tissue spaces as revealed by scanning electron microscopy. Cell Tissue Res. 148, 111–125 (1974)
Muto, M.: A scanning electron microscopic study on endothelial cells and Kupffer cells in rat liver sinusoids. Arch. Histol. Jpn. 37, 369–386 (1975)
Nopanitaya, W., Grisham, J.W.: Scanning electron microscopy of mouse intrahepatic structures. Exp. Mol. Pathol. 23, 441–458 (1975)
Nopanitaya, W., Grisham, J.W., Carson, J.L., Dotson, M.M.: Surface features of cirrhotic liver. Virchows Arch. [Pathol. Anat.] 372, 97–108 (1976)
Ogawa, K.T., Minase, T., Enomoto, K., Onoe, T.: Ultrastructure of fenestrated cells in the sinusoidal wall of rat liver after perfusion fixation. Tohoku J. Exp. Med. 110, 89–101 (1973)
Orci, L.A., Matter, A., Rouiller, C.: A comparative study of freeze-etch replicas and thin sections of rat liver. J. Ultrastruct. Res. 35, 1–19 (1971)
Rappaport, A.M.: The microcirculatory hepatic unit. Microvasc. Res. 6, 212–228 (1973)
Staehelin, L.A., Hull, B.E.: Junctions between living cells. Sci. Am. 238, 140–153 (1978)
Wisse, E.: An electron microscopic study of the fenestrated endothelial lining of rat liver sinusoids. J. Ultrastruct. Res. 31, 125–150 (1970)
Yamamoto, T.: Some observations on the fine structure of the terminal biliary passages in the goldfish liver. Anat. Rec. 142, 293 (1962)
Yamamoto, T.: Some observations on the fine structure of the intrahepatic biliary passages in goldfish (Carassius auratus). Z. Zellforsch. 65, 319–330 (1965)
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Nopanitaya, W., Carson, J.L., Grisham, J.W. et al. New observations on the fine structure of the liver in goldfish (Carassius auratus). Cell Tissue Res. 196, 249–261 (1979). https://doi.org/10.1007/BF00240100