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β-Oxidation enzymes in the mitochondria of Arum and oilseed rape

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β-Oxidation enzymes were detected both in the mitochondria and microbodies of Arum maculatum L. spadices and Brassica napus L. seeds. It is apparent that the mitochondrial membrane barrier, which remains intact after sucrose-density-gradient centrifugation, prevents rapid access of acyl-GoA substrates to matrix βoxidation tes. Thus intact mitochondria showed little β-oxidation enzyme activity. Rupturing of the mitochondrial membrane allowed rapid access of acyl CoAs to matrix sites. Consequently, in ruptured mitochondria, high β-oxidation enzyme activities were measured.

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  1. Aebi, H. (1974) Catalase. In: Methods of enzymatic analysis, vol. 2, pp. 673–684, Bergmeyer, H.E., ed. Verlag Chemie, Weinheim

  2. ap Rees, T. (1980) Assessment of the contributions of metabolic pathways to plant respiration. In: The biochemistry of plants, a comprehensive treatise, vol. 2. pp. 1–29, Stumpf, P.K., Conn, E.E., eds. Academic Press, New York

  3. Beevers, H. (1979) Microbodies in higher plants. Annu. Rev. Plant Physiol. 30, 159–193

  4. Beevers, H., Breidenbach, R.W. (1974) Glyoxysomes. Methods Enzymol. 31, 565–571

  5. Bieber, L.L., Krahling, J.B., Clarke, P.R.H., Valkner, K.J., Tolbert, N.E. (1981) Carnitine acyltransferases in rat liver peroxisomes. Arch. Biochem. Biophys. 211, 599–604

  6. Burgess, N., Thomas, D.R. (1986) Carnitine acetyltransferase in pea cotyledon mitochondria. Planta. 167, 58–65

  7. Burgess, N., Beakes, G.W., Thomas, D.R. (1985) Separation of mitochondria from microbodies of Pisum sativum (L. cv. Alaska) cotyledons. Planta 166, 151–155

  8. Chapman, D., Leslie, R.B. (1970) Structure and function of phospholipids in membranes. In: Membranes of mitochondria and chloroplasts, pp. 91–115, Racker, E., ed. Van Nostrand Reinold Company, New York London

  9. Cooper, T.G., Beevers, H. (1969a) Mitochondria and glyoxysomes from castor bean endosperm. Enzyme constituents and catalytic capacity. J. Biol. Chem. 244, 3507–3513

  10. Cooper, T.G., Beevers, H. (1969b) β-oxidation in glyoxysomes from castor bean endosperm. J. Biol. Chem. 244, 3514–3520

  11. Donaldson, R.P., Tully, R.E., Young, O.A., Beevers, H. (1981) Organelle membranes from germinating castor bean endosperm. II. Enzymes, cytochromes and permeability of the glyoxysome membrane. Plant Physiol. 67, 21–25

  12. Feierabend, J., Beevers, H. (1972) Developmental studies on microbodies in wheat leaves. 1. Conditions influencing enzyme development. Plant Physiol. 49, 28–32

  13. Galliard, T. (1980) Degradation of acyl lipids: hydrolytic and oxidative enzymes. In: The biochemistry of plants, a comprehenive treatise, vol. 4, pp. 85–116, Stumpf, P.K., Conn, E.E., eds. Academic Press, New York

  14. Gerbling, H., Gerhardt, B. (1988) Carnitine acyltransferase activity of mitochondria from mung bean hypocotyls. Planta 174, 90–93

  15. Gerhadt, B., ed. (1978) Microbodies/Peroxisomen pflanzlicher Zellen. (Cell Biology Monographs, vol. 5). Springer, Vienna New York

  16. Gerhardt, B. (1981) Enzyme activities of the β-oxidation pathway in spinach leaf peroxisomes. FEBS Lett. 126, 71–73

  17. Gerhardt, B. (1983) Localization of β-oxidation enzymes in peroxsomes isolated from non-fatty plant tissues. Planta 159, 238–246

  18. Hill, R.L., Bradshaw, R.A. (1969) Fumarase. Methods Enzymol. 13, 91–99

  19. Hutton, D., Stumpf, P.K. (1969) Fat metabolism in higher plants. XXXVII. Characterization of the β-oxidation systems from maturing and germinating castor bean seeds. Plant Physiol. 44, 508–516

  20. Kindl, H. (1987) β-Oxidation of fatty acids by specific organelles. In: The biochemistry of plants, a comprehensive treatise, vol. 9, pp. 31–52, Stumpf, P.K., Conn, E.E., eds. Academic Press, New York

  21. Macey, M.J.K. (1983) β-oxidation and associated enzyme activities in microbodies from germinating peas. Plant Sci. Lett. 30, 53–60

  22. Macey, M.J.K., Stumpf, P.K. (1983) β-oxidation enzymes in microodies from tubers of Helianthus tuberosus. Plant Sci. Lett. 28, 207–212

  23. Markwell, M.A.K., Haas, S.M., Bieber, L.L., Tolbert, N.E. (1978) A modification of the Lowry procedure to simplify protein determination in membrane and lipo-protein samples. Anal. Biochem. 87, 206–210

  24. Mazliak, P. (1973) Lipid metabolism in plants. Annu. Rev. Plant Physiol. 24, 287–310

  25. McNeil, P.H., Thomas, D.R. (1976) The effect of carnitine on palmitate oxidation by pea cotyledon mitochondria. J. Exp. Bot. 27, 1163–1180

  26. Miernyk, J.A., Trelease, R.N. (1981) Control of enzyme activities in cotton cotyledons during maturation and germination. IV. β-oxidation. Plant Physiol. 67, 341–346

  27. Nishimura, T., Saito, T., Tomita, K. (1978) Purification and properties of β-Ketothiolase from Zoogloea ramigera. Arch. Microbiol. 116, 21–27

  28. Olsen, J.A., Huang, A.H.C. (1988) Glyoxysomal acyl CoA synthetase and oxidase from germinating elm, rape and maize seed. Phytochemistry 27, 1601–1603

  29. Overath, P., Raufuss, E.M., Stoffel, M., Ecker, W. (1967) The induction of enzymes of fatty acid degradation in Escherichia coli. Biochem. Biophys. Res. Commun. 29, 28–33

  30. Panter, R.A., Mudd, J.B. (1969) Carnitine levels in some higher plants. FEBS Lett. 5, 169–170

  31. Panter, R.A., Mudd, J.B. (1973) Some aspects of carnitine metaboism in avocado (Persea americana). Biochem. J. 134, 655–658

  32. Thomas, D.R., McNeil, P.H. (1976) The effect of carnitine on the oxidation of saturated fatty acids by pea cotyledon mitochondria. Planta 132, 61–63

  33. Thomas, D.R., Wood, C. (1986) The two β-oxidation sites in pea cotyledons. Carnitine palmitoyltransferase: location and function in pea mitochondria. Planta 168, 261–266

  34. Thomas, D.R., Noh Hj Mil, M., Cooke, R.J., Yong, B.C.S., Ariffin, A., McNeil P.H., Wood, C. (1982) The synthesis of palmitoylcarnitine by etio-chloroplasts of greening barley leaves. Planta 154, 60–65

  35. Tolbert, N.B. (1981) Metabolic pathways in peroxisomes and glyoxysomes. Annu. Rev. Biochem. 50, 133–157

  36. Walker, D.B., Gysi, J., Sternberg, L., De Niro, M.J. (1983) Direct respiration of lipids during heat production in the inflorescence of Philodendron selloum. Science 220, 419–421

  37. Wood, C., Noh Hj Jalil, M., Ariffin, A., Yong, B.C.S., Thomas, D.R. (1983) Carnitine short-chain acyltransferase in pea mitochondria. Planta 158, 175–178

  38. Wood, C., Noh Hj Jalil, M., McLaren, I., Yong, B.C.S., Ariffin, A., McNeil, P.H., Burgess, N., Thomas, D.R. (1984) Carnitine long-chain acyltransferase and oxidation of palmitate, palmitoyl coenzyme A and palmitoylcarnitine by pea mitochondria preparations. Planta 161, 255–260

  39. Wood, C., Burgess, N., Thomas, D.R. (1986) The dual location of β-oxidation enzymes in germinating pea cotyledons. Planta 167, 54–57

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Correspondence to D. R. Thomas.

Additional information

C. Masterson thanks the Science and Engineering Research Council for the award of a postgraduate student maintenance grant. D.R. Thomas and C. Wood thank their relatives for continuing financial support. The authors also thank West Cumberland Farmers Ltd., Hexham, UK for their gift of oilseed rape seeds.

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Masterson, C., Wood, C. & Thomas, D.R. β-Oxidation enzymes in the mitochondria of Arum and oilseed rape. Planta 182, 129–135 (1990). https://doi.org/10.1007/BF00239994

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Key words

  • Arum
  • Brassica (β-oxidation)
  • β-Oxidation
  • (enzyme location)
  • Microbody
  • Mitochondrion