Advertisement

Springer Nature is making Coronavirus research free. View research | View latest news | Sign up for updates

PO2-profiles in hippocampal slices of the guinea pig

  • 78 Accesses

  • 54 Citations

Summary

For a detailed analysis of the oxygen supply of hippocampal slices, tissue PO2 (Pt,o 2) was recorded polarographically in the neural layers of thick and thin slice preparations from the guinea pig. The experiments showed that the Pt,o2-gradients were extremely steep in the outer zones of vital slices. In an air equilibrated salt solution the surface PO2 was reduced to less than 50% within ca. 25 μm. Minimum values were measured at a depth of ca. 150 μm. A rise of temperature lowered the oxygen supply in the deeper layers of the excised tissue. An elevation of the surface PO2 hardly improved Pt,o 2 in the deep structures, because the O2-consumption of the hippocampal slices increased with rising PO2.

This is a preview of subscription content, log in to check access.

References

  1. Acker H, Keller H-P, Lubbers DW, Bingmann D, Schulze H, Caspers H (1973) The relationship between neuronal activity of chemoreceptor fibers and tissue PO2 and blood pressure. Pfluegers Arch 343: 287–296

  2. Acker H, Heinrich R, Lübbers DW, Bingmann D, Caspers H (1977) The relation between tissue PO2 in the cat carotid body and chemoreceptor activity under hyperoxic conditions. Proc of the International Union of Physiological Sciences 13: 5

  3. Andersen P, Bliss TVP, Skrede KK (1971) Lamellar organization of hippocampal excitatory pathways. Exp Brain Res 13: 222–238

  4. Bak I, Misgeld U, Weiler M, Morgen E (1980) The preservation of nerve cells in rat neostriatal slices maintained in vitro: a morphological study. Brain Res 197: 341–353

  5. Bingmann D, Kolde G (1979) Burst activity elicited in hippocampal slices by a rise of PO2. Pfluegers Arch 382: 38

  6. Brierly JB (1976) Cerebral hypoxia. In: Blackwood W, Corsellis J (eds) Greenfield's Neuropathology. Arnold, London pp 43–85

  7. Burg von R, Lijoi A, Smith C (1979) Oxygen consumption of rat tissue slices exposed to methylmercury in vitro. Neurosci Lett 14: 309–314

  8. Clark DK, Erdmann W, Halsey JH, Strong F (1978) Oxygen diffusion, conductivity and solubility coefficients in the micro area of the brain. In: Silver IA, Erecinska M, Sicher H (eds) Oxygen transport to tissue III. Plenum, New York pp 697–704

  9. Dingledine R, Dodd J, Kelly JS (1980) The in vitro brain slice as a useful neurophysiological preparation for intracellular recording. J Neurosci Meth 2: 323–362

  10. Farr DA, Fuhrmann FA (1965) Role of diffusion of oxygen in the respiration of tissues at different temperatures. J Appl Physiol 20: 637–646

  11. Fuhrmann FA, Field J (1945) Factors determining the metabolic rate of excises liver tissue. Arch Biochem 6: 337–349

  12. Ganfield RA, Nair P, Whalen WJ (1970) Mass transfer, storage and utilization of O2 in cat cerebral cortex. Am J Physiol 219: 814–821

  13. Garthwaite J, Woodhams PL, Collius MJ, Balazs R (1979) On the preparation of brain slices: morphology and cyclic nucleotides. Brain Res 173: 373–377

  14. Grote J, Süsskind R, Vaupel P (1978) Oxygen diffusion constants D and K of tumor tissue and their temperature dependence: In: Silver IA, Erecinska M, Bicher H (eds) Oxygen transport to tissue III. Plenum, New York pp 361–365

  15. Hansen AJ, Jahnsen H, Hounsgaard JB (1979) Influence of hypoxia on hippocampal nerve cells in vitro. Acta Physiol Scand [Suppl] 473: 55

  16. Harvey JA, Scholfield CN, Brown DA (1974) Evoked surface- positive potentials in isolated mammalian olfactory cortex. Brain Res 76: 235–245

  17. Jenkins LW, Povlishock JT, Becker DP, Miller JD, Sullivan HG (1979) Complete cerebral ischemia. An ultrastructural study. Acta Neuropathol (Berl) 48: 113–125

  18. Lehmenkühler A, Caspers H, Speckmann E-J (1976) A method for simultaneous measurement of bioelectric activity and local tissue PO2 in the CNS. In: Grote J, Reneau D, Thews G (eds) Oxygen transport to tissue II. Plenum, New York pp 3–7

  19. Lehmenkühler A, Zidek W, Staschen M, Caspers H (1981) Cortical pH and pCa in relation to DC potential shift during spreading depression and asphyxiation. In: Vyklicky L, Sykova E, Huik P (eds) Ion-selective microelectrodes and their use in excitable tissues. Plenum, New York pp 225–230

  20. Lensing J, Sasse L (1978) Gleichzeitiges Messen des Sauerstoff- partialdrucks und bioelektrischer Potentiale in lebendem Gewebe. Elektronik 4: 91–94

  21. Lierse W (1963) Die Kapillardichte im Rhinencephalon verschiedener Wirbeltiere und des Menschen. In: Bargmann W, Schade JP (eds) Progress in brain research, Vol. 3, The rhinencephalon and related structures. Elsevier, Amsterdam pp 230–236

  22. Lipton P, Wittingham TS (1979) The effect of hypyoxia on evoked potentials in the vitro hippocampus. J Physiol (London) 287: 427–438

  23. Lübbers DW (1973) Local tissue PO2: its measurement and meaning In: Kessler M, Bruley DF, Clark LC, Lubbers DW, Silver IA, Strauss J (eds) Oxygen suppley. Urban & Schwarzenberg, München Berlin Wien pp 151–155

  24. Lynch G, Schubert P (1980) The use of in vitro brain slices for multidisciplinary studies of synaptic function. Ann Rev Neurosci 3: 1–22

  25. Metzger H, Heuber-Metzger S, Steinacker A, Strüber J (1980) Staining PO2 measurement sites in the rat brain cortex and quantitative morphometry of the surrounding capillaries. Pfluegers Arch 388: 21–27

  26. Morris ME (1974) Hypoxia and extracellular potassium activity in the guinea pig cortex. Can J Physiol Pharmacol 5: 872–882

  27. Scholfield CN (1978) Electrical properties of neurons in the olfactory cortex slices in vitro. J Physiol (London) 275: 535–546

  28. Schwarzkroin PA (1975) Characteristics of CA 1 neurons recorded intracellularly in the hippocampal in vitro slice preparation. Brain Res 85: 423–436

  29. Silver JA (1977) Ion fluxes in hypoxic tissues. Microvasc Res 13 409–420

  30. Skredde KK, Westgaard RH (1971) The transverse hippocampal tissue: a well defined cortical structure maintained in vitro. Brain Res 35: 589–593

  31. Smith RH, Guilbeau EJ, Renau EY (1977) The oxygen tension field within a discrete volume of cerebral cortex. Microvasc Res 13: 233–244

  32. Speckmann E-J, Caspers H (1974) The effects of O2 and CO2 tensions in the nervous tissue on neuronal activity and DC potentials. In: Rémond A (ed) Handbook of Electroence-phalography and Clinical Neurophysiology II C. Elsevier, Amsterdam London New York pp 71–89

  33. Tayler TJ (1980) Brain slice preparation: hippocampus. Brain Res Bull 5: 391–403

  34. Thews G (1960) Ein Verfahren zur Bestimmung des O2-Diffusionskoeffizienten, der O2-Leitfähigkeit und des Löslichkeitskoeffizienten im Gehirngewebe. Pfluegers Arch 271: 227–244

  35. Tsacopoulos M, Lehmenkühler A (1977) A double-barrelled Pt-microelectrode for simultaneous measurement of PO2 and bioelectrical activity in excitable tissues. Experientia 33: 1337–1338

  36. Tsacopoulos M, Poitry S, Borsellino A (1981) Diffusion and consumption of oxygen in the superfused retina of the drone in darkness. J Gen Physiol 77: 601–628

  37. Vyskocil F, Kriz N, Bures J (1972) Potassium-selective microelectrodes used for measuring the extracellular brain potassium during spreading depression and anoxic depolarization in rats. Brain Res 39: 255–259

  38. Warburg O (1923) Versuche an überlebendem Carcinomgewebe. Biochem Z 142: 317–333

  39. Yamamoto C (1972) Intracellular study of seizure-like afterdischarges elicited in thin hippocampal sections in vitro. Exp Neurol 35: 154–164

  40. Yamamoto C, Kurokawa M (1970) Synaptic potentials recorded in brain slices and their modification by changes in the level of tissue ATP. Exp Brain Res 10: 159–170

Download references

Author information

Correspondence to Dr. D. Bingmann.

Additional information

Dedicated to Professor Dr. H. Caspers on the occasion of his 60th birthday

Supported by DFG (Sp 108/6)

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Bingmann, D., Kolde, G. PO2-profiles in hippocampal slices of the guinea pig. Exp Brain Res 48, 89–96 (1982). https://doi.org/10.1007/BF00239575

Download citation

Key words

  • Hippocampal slices
  • PO2-profiles
  • Temperature
  • PO2-dependent oxygen consumption