In rats anaesthetized with urethane, push pull cannulae were stereotaxically introduced in the hippocampus (bilaterally) and Zn2+ assayed in the perfusate by atomic absorption spectrophotometry. When the cannula was located in the immediate vicinity of the mossy fiber zone, both spontaneous and K+ evoked release of Zn2+ were observed, this release was associated with a reduction in the histologically demonstrable Zn2+ as assessed by means of the Timm's stain. Neither spontaneous nor evoked release of Zn2+ was observed when the cannula was located in the medial part of CA1, the fimbria or the thalamus. These observations suggest that Zn2+ is released in the mossy fiber zone.
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Charton, G., Rovira, C., Ben-Ari, Y. et al. Spontaneous and evoked release of endogenous Zn2+ in the hippocampal mossy fiber zone of the rat in situ. Exp Brain Res 58, 202–205 (1985). https://doi.org/10.1007/BF00238969
- Push pull
- Mossy fibers