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Genotypic and media factors affecting stabilization of polyembryogenic cultures in norway spruce (Picea abies (L.) Karst.)

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Abstract

Embryonal-suspensor mass lines (ESM lines) from mature embryos of three open-pollinated families of Norway spruce [Picea abies (L.) Karst.] were stabilized on several culture media varying in nutrient composition. A pattern of relationship between parameters of polyembryogenic culture performance and medium composition was genetically determined. Stabilized ESM lines could be classified into two phenovariants: (i) lines of homogenous ESM composed entirely of somatic proembryos, and (ii) lines of heterogeneous ESM composed of a mixture of nonembryogenic callus and aggregates of somatic proembryos. The formation of one or the other phenovariant was independent of genotype, but strongly determined by the medium composition.

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Abbreviations

ABA:

abscisic acid

BA:

6-benzyladenine

ESM:

embryonal-suspensor mass

N:

nitrogen

NAA:

α-naphthyl-1-acetic acid

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Communicated by H. Lörz

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Bozkov, P.V. Genotypic and media factors affecting stabilization of polyembryogenic cultures in norway spruce (Picea abies (L.) Karst.). Plant Cell Reports 14, 389–392 (1995). https://doi.org/10.1007/BF00238603

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Keywords

  • Medium Composition
  • Nutrient Composition
  • Mature Embryo
  • Media Factor
  • Culture Performance