Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

A novel life cycle arising from leaf segments in plants regenerated from horseradish hairy roots

  • 34 Accesses

  • 6 Citations

Summary

Horseradish (Armoracia rusticana) hairy root clones were established from hairy roots which were transformed with the Ri plasmid in Agrobacterium rhizogenes 15834. The transformed plants, which were regenerated from hairy root clones, had thicker roots with extensive lateral branches and thicker stems, and grew faster compared with non-transformed horseradish plants. Small sections of leaves of the transformed plants generated adventitious roots in phytohormone-free G (modified Gamborg's) medium. Root proliferation was followed by adventitious shoot formation and plant regeneration. Approximately twenty plants were regenerated per square centimeter of leaf. The transformed plants were easily transferable from sterile conditions to soil. When leaf segments of the transformed plants were cultured in a liquid fertilizer under non-sterile conditions, adventitious roots were generated at the cut ends of the leaves. Adventitious shoots were generated at the boundary between the leaf and the adventitious roots and developed into complete plants. This novel life cycle arising from leaf segments is a unique property of the transformed plants derived from hairy root clones.

This is a preview of subscription content, log in to check access.

References

  1. Bergmeyer HU (ed.) (1974) Methods of Enzymatic Analysis, Academic Press, New York London

  2. Bradford MM (1976) Anal Biochem. 72: 248–254

  3. Chilton MD, Tepfer DA, Petit A, David C, Casse-Delbart F, Tempe J (1982) Nature 295: 432–434

  4. Fujii I, Hirayama N, Miike A (1993) Acta Cryst. C49: 1540–1541

  5. Lambert C, Tepfer D (1991) Bio/Technology 9: 80–83

  6. Mano Y, Nabeshima S, Matsui C, Ohkawa H (1986) Agric. Biol. Chem. 50: 2715–2722

  7. Mano Y, Ohkawa H, Yamada Y (1989) Plant Science 59: 191–201

  8. Mano Y (1993) In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry, vol 22, Springer-Verlag, Berlin Heidelberg, pp 190–201

  9. Noda T, Tanaka N, Mano Y, Nabeshima S, Ohkawa H, Matsui C (1987) Plant Cell Reports 6: 283–286

  10. Ooms G, Karp A, Burrell MM, Twell D, Roberts J (1985) Theor. Appl. Genet. 70: 440–446

  11. Phelep M, Petit A, Martin L, Duhoux E, Tempe J (1991) Bio/Technology 9: 461–466

  12. Saitou T, Kamada H, Harada H (1991) Plant Science 75: 195–201

  13. Saitou T, Kamada H, Harada H (1992) Plant Physiology 99: 1336–1341

  14. Saitou T, Tachikawa Y, Kamada H, Watanabe M, Harada H (1993) Planta 189: 590–592

  15. Tepfer DA (1984) Cell 37: 959–967

  16. Zambryski P, Tempe J, Schell J (1989) Cell 56: 193–201

Download references

Author information

Additional information

Communicated by A. Komamine

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Mano, Y., Matsuhashi, M. A novel life cycle arising from leaf segments in plants regenerated from horseradish hairy roots. Plant Cell Reports 14, 370–374 (1995). https://doi.org/10.1007/BF00238599

Download citation

Key words

  • Agrobacterium rhizogenes
  • Hairy roots
  • Ri plasmid
  • Transformation
  • Armoracia rusticana
  • Regeneration