The mouse brain auditory pathway has been dissected into five regions: geniculate bodies, posterior colliculi, superior olives, cochlear nuclei, and cochleas. The following analyses were made in these regions and in the auditory cortex: protein, glutamate, γ-aminobutyrate, taurine, choline acetyltransferase, and glutamate decarboxylase.
Taurine levels (nmol · mg of protein-1) were highest in cortex (93) and geniculate bodies (60) and lowest in the cochlear nuclei (27) and cochleas (29).
Concentrations of γ-aminobutyrate (same units) were highest in the geniculate bodies (28), low in the superior olives and cochlear nuclei (9 to 10), and undetectable in the cochleas. The distribution of glutamate decarboxylase activity reflected that of γ-aminobutyrate.
The activities of choline acetyltransferase (nmol · of acetylcholine synthesized · h · -1 mg of protein-1) were highest in the superior olives (60) and low in the cochleas (3).
These results are interpreted as biochemical support for previous physiological and pharmacological identification of the olivo-cochlear bundle as cholinergic and the cochlear-nucleus neurones as non-cholinergic. The results also provide further evidence for a role of GABA in the posterior colliculi, but not in the cochleas.
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Baxter, C.F.: Assay of γ-aminobutyric acid and enzymes involved in its metabolism. In: Methods of neurochemistry, R. Fried (Ed.), Vol. 3, pp. 3–73. New York: Dekker 1972
Bures, J., Petran, M., Zachar, J.: Electrophysiological methods in biological research, 3rd ed. New York: Academic Press 1967
Comis, S.D., Davies, W.E.: Acetylcholine as a transmitter in the cat auditory system. J. Neurochem. 16, 423–429 (1969)
Curtis, D.R., Johnston, G.A.R.: Aminoacid transmitters in the mammalian central nervous system. Ergeb. Physiol. 69, 97–188 (1974)
Desmedt, J.E., Robertson, D.: Ionic mechanism of the efferent olivocochlear inhibition studied by cochlear perfusion in the cat. J. Physiol. (Lond.) 247, 407–428 (1975)
Duffy, T.E., Nelson, S.R., Lowry, O.H.: Cerebral carbohydrate metabolism during acute hypoxia and recovery. J. Neurochem. 19, 959–977 (1972)
Ebel, A., Hermett, J.C., Mandel, P.: Comparative study of acetylcholinesterase and choline acetyltransferase enzyme activity in brain of DBA and C57 mice. Nature (New Biol.) 242, 56–57 (1973)
Fisher, S.K., Davies, W.E.: The presence and role of γ-aminobutyric acid (GABA) in the guinea pig auditory system. J. Neurochem. 27, 1145–1155 (1976)
Folbergrova, J., Passonneau, J.V., Lowry, O.H., Schulz, D.W.: Glycogen, ammonia and related metabolites in the brain during seizures evoked by methionine sulphoximine. J. Neurochem. 16, 191–203 (1969)
Fonnum, F.: Recent developments in biochemical investigations of cholinergic transmission. Brain Res. 62, 497–507 (1973)
Fonnum, F.: Rapid radiochemical method for the determination of choline acetyl transferase. J. Neurochem. 24, 407–410 (1975)
Galley, N., Klinke, R., Oertel, W., Pause, M., Storch, W.H.: The effect of intracochlearly administered acetylcholine-blocking agents on the efferent synapses of the cochlea. Brain Res. 64, 55–63 (1973)
Goldberg, A.M., Kaita, A.A., McCaman, R.E.: Microdetermination of choline acetyl transferase: a comparison of reinecke vs periodide precipitation. J. Neurochem. 16, 823–824 (1969)
Graham, L.T., Aprison, M.H.: Fluorometric determination of aspartate, glutamate and γ-aminobutyrate in nerve tissue using enzymic methods. Anal. Biochem. 15, 487–497 (1966)
Haldeman, S., Huffman, R.D., Marshall, K.C., McLennan, H.: The antagonism of the glutamate-induced and synaptic excitations of thalamic neurons. Brain Res. 39, 419–525 (1972)
Jasser, A., Guth, P.S.: The synthesis of acetylcholine by the olivecochlear bundle. J. Neurochem. 20, 54–53 (1973)
Kennedy, A.J., Voaden, M.J.: Free aminoacids in the photoreceptor of the frog retina. J. Neurochem. 23, 1093–1095 (1974)
King, L.J., Carl, J.L., Lao, L.: Brain aminoacids during convulsions. J. Neurochem. 22, 307–309 (1974)
Klinke, R., Galley, N., Pause, M., Storch, W.J.: Tetanus toxin blocks the efferent endings in the cochlea. Brain Res. 49, 447–450 (1973)
Kobayashi, R.M., Brownstein, M., Saavedra, J.M., Palkovits, M.: Choline acetyl transferase content in discrete regions of the rat brain stem. J. Neurochem. 24, 637–640 (1975)
Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–275 (1951)
McIlwain, H., Bachelard, H.S.: Biochemistry and central nervous system, p. 172. London: Churchill-Livingstone 1971
McLennan, H.: Actions of excitatory aminoacids and their antagonism. Neuropharmacology 13, 449–454 (1974)
Obata, K.: The inhibitory action of γ-aminobutyric acid, a probable synaptic transmitter. Int. Rev. Neurobiol. 15, 167–187 (1972)
Oja, S.S., Lähdesmäki, P.: Is taurine an inhibitory neurotransmitter? Med. Biol. 52, 138–143 (1974)
Roberts, E.: Disinhibition as an organizing principle in the nervous system. The role of GABA. Adv. Neurol. 5, 127–143 (1974a)
Roberts, E.: γ-Aminobutyric acid and nervous system function — a perspective. Biochem. Pharmacol. 23, 2637–2649 (1974b)
Ross, M.D.: Auditory pathway of the epileptic waltzing mouse: a comparison of the acoustic pathways of the normal mouse with those of the totally deaf epileptic waltzer. J. Comp. Neurol. 119, 317–338 (1962)
Schrier, B.K., Shuster, L.: A simplified radiochemical assay for CAT. J. Neurochem. 14, 977–985 (1967)
Sidman, R.L., Angevine, J.B., Pierce, E.T.: Atlas of the mouse brain and spinal cord. Cambridge: Harvard University Press 1971
Spench, H.J.: Antagonism and cortical excitation of striatal neurons by glutamic acid diethyl ester: Evidence for glutamic acid as an excitatory transmitter in the rat striatum. Brain Res. 102, 91–102 (1976)
Tachibana, M., Kuriyama, K.: γ-aminobutyric acid in the lower auditory pathway of the guinea pig. Brain Res. 69, 370–374 (1974)
Tebecis, A.K.: Transmitters and identified neurons in the mammalian central nervous system. Bristol: Scientechnica 1974
Van Gelder, N.M.: Glutamate dehydrogenase, GAD and GABA amino transferase in epileptic mouse cortex. Can. J. Physiol. Pharmacol. 52, 952–959 (1974)
Watanabe, T.: Effects of picrotoxin on the two-tone inhibition of auditory neurons in the cochlear nucleus. Brain Res. 28, 586–590 (1971)
Watanabe, T., Simada, Z.: Picrotoxin: effect on collicular auditory neurons. Brain Res. 28, 582–585 (1971)
Wenthold, R.J.: Glutamic acid and aspartic acid in subdivisions of the cochlear nucleus after auditory nerve lesion. Brain Res. 143, 544–548 (1978)
Wenthold, R.J., Gulley, R.L.: Aspartic acid and glutamic acid levels in the cochlear nucleus after auditory nerve lesion. Brain Res. 138, 111–123 (1977)
Wu, J.Y., Matsuda, T., Roberts, E.: Purification and characterization of glutamate decarboxylase from mouse brain. J. Biol. Chem. 248, 3029–3034 (1973)
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Contreras, N.E.I.R., Bachelard, H.S. Some neurochemical studies on auditory regions of mouse brain. Exp Brain Res 36, 573–584 (1979). https://doi.org/10.1007/BF00238524
- Auditory system
- Amino acids