Mouse NIH 3T3 fibroblasts were starved by serum depletion and subsequently restimulated by addition of serum. Histone acetylation and histone synthesis were studied from the beginning of starvation to the point where most of the cells were in S-phase utilizing electrophoretic and fluorographic techniques. We found that the major part of histone acetylation and histone synthesis occurs during S-phase but that also in the absence of DNA synthesis there are significant changes in the acetylation and synthesis rates of the core histones which occur during the first 6 hours of serum stimulation of quiescent cells, and between 24 and 48 hours of serum starvation.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Vidali G, Ferrari N, Pfeffer U: Other post-translational modifications of proteins. In: Advances in Exp. Medicine and Biology. Plenum Publ., New York, 1988, 231: 583–597
Chen TA, Allfrey VG: Rapid and reversible changes in nucleosome structure accompany the activation, repression and superinduction of murine fibroblast protooncogenes c-fos and c-myc. Proc Natl Acad Sci USA 84: 5252–5256, 1987
Weintraub H, Palter K, Van Lente F: Histones H2a, H2b, H3 and H4 form a tetrameric complex in solution of high salt. Cell 6: 85–110, 1975
Knosp O, Redl B, Puschendorf B: Biochemical characterization of chromatin fractions from induced and uninduced friend erythroleukemia cells. Mol Cell Biochem 89: 37–46, 1989
Wu RS, Bonner WM: Separation of basal histone synthesis from S-phase histone synthesis in dividing cells. Cell 27: 321–330, 1981
Wu RS, Tsai, Bonner WM: Patterns of histone variant synthesis can distinguish Go from Gl cells. Cell 31: 443–452, 1982
Loidl P: Towards an understanding of the biological function of histone acetylation. Febs Lett 227, 2: 91–95, 1988
Jackson V, Shires A, Tauphaichtitr N, Chalkley R: Modifications of histones immediately after synthesis. J Mol Biol 104: 471–483, 1976
Worcel A, Han S, Wong ML: Assembly of newly replicated chromatin. Cell 15: 969–977, 1978
Waterborg IH, Matthews HR: Patterns of histone acetylation in the cell cycle of Physarum polycephalum. Biochem 22: 1489–1496, 1983
Allis CD, Chicoine LG, Richman R, Schulman JG: Deposition-related histone acetylation in micronuclei of conjugating Tetrahymena. Proc Natl Acad Sci USA 82: 8048–8052, 1985
About this article
Cite this article
Knosp, O., Talasz, H. & Puschendorf, B. Histone acetylation and histone synthesis in mouse fibroblasts during quiescence and restimulation into S-phase. Mol Cell Biochem 101, 51–58 (1991). https://doi.org/10.1007/BF00238437
- fibroblasts (mouse)
- histone acetylation
- histone synthesis