Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Association of Thy-1 cell surface differentiation antigen with certain connective tissues in vivo


The cell surface differentiation antigen, Thy-1, has been demonstrated by immunofluorescence to be associated with some collagen-based connective tissue. In the rat, the basement membrane of kidney collecting tubules and of certain blood vessels, reticulin of the lamina propria, loose connective tissue in the dermis, and collagen fibres within certain nerve cell tumours, bear the antigen. Other, apparently similar, connective tissue (especially that of muscle and liver) lacked Thy-1. In the mouse, only the connective tissue of the skin was found to bear the antigen. The possible origin of this extracellular Thy-1, and its implications for the function of the molecule, are discussed.

This is a preview of subscription content, log in to check access.


  1. Acton RT, Morris RJ, Williams AF (1974) Estimation of the amount and tissue distribution of rat Thy-1.1 antigen. Eur J Immunol 4:598–602

  2. Bailey AJ, Shellswell GB, Duance VC (1979) Identification and change of collagen types in differentiating myoblasts and developing chick muscle. Nature 278:67–69

  3. Baldwin RW, Price MR Cell membrane associated antigens in chemical carcinogenesis. Biomembranes 8:89–129

  4. Barclay AN, Letarte-Muirhead M, Williams AF (1975) Purification of the Thy-1 molecule from rat brain. Biochem J 151:699–706

  5. Barclay AN, Letarte-Muirhead M, Williams AF, Faulkes RA (1976) Chemical characterization of the Thy-1 glycoproteins from the membranes of rat thymocytes and brain. Nature 263:563–567

  6. Bourguignon LWE, Hyman R, Trowbridge I, Singer SJ (1978) Localization of T25 glycoprotein in wild-type and Thy-1 mutant cells by immunofluorescence and immunoelectron microscopy. J Exp Med 147:1348–1354

  7. Boyd RL, Ward HA, Muller HK (1976) Antiserum specific for reticulin of the bursa of Fabricius. Int Arch Allerg Appl Immunol 50:129–132

  8. Boyse EA, Old LJ (1969) Some aspects of normal and abnormal cell surface genetics. Ann Rev Genetics 3:269–290

  9. Douglas T (1972) Occurrence of a theta-like antigen in rats. J Exp Med 136:1054–1062

  10. Duance VC, Restall DJ, Beard H, Bourne FJ, Bailey AJ (1977) The location of three collagen types in skeletal muscle. FEBS Letts 79:248–252

  11. Ferwerda W, Loon CMI, Feltkamp-Vroom TM (1978) The specificity of the antigenic determinants of bovine renal tubular basement membrane. In: Kefalides NA (ed) Biology and Chemistry of Basement Membranes. Academic Press, New York 403–415

  12. Gabella F (1972) Fine structure of the myenteric plexus in the guinea-pig ileum. J Anat 111:69–97

  13. Goldschneider I, Gordon LK, Morris RJ (1978) Demonstration of Thy-1 antigen on pluripotent hemopoietic stem cells in the rat. J Exp Med 148:1351–1366

  14. Gridley MF (1951) Modification of silver impregnation method of staining reticular fibers. Amer J Clin Pathol 21:897–899

  15. Kefalides NA (1978) Current status of chemistry and structure of basement membranes. In: NA Kefalides ed, Biology and Chemistry of Basement Membranes, Academic Press, pp 215–228, New York

  16. Kuchel PW, Campbell DG, Barclay AN, Williams AF (1978) Molecular weights of the Thy-1 glycoproteins from rat thymus and brain in the presence and absence of deoxycholate. Biochem J 169:411–417

  17. Lebacq A-M, Ritter MA (1979) B-cell precursors in early chicken embryos. Immunology 37:123–134

  18. Lennon VA, Unger M, Dulbecco R (1978) Thy-1: a differentiation marker of potential mammary myoepithelial cells in vitro. Proc Natl. Acad Sci (USA) 75:6093–6097

  19. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275

  20. Mayne R, Vail MS, Miller EJ (1978) Characterization of the collagen chains synthesised by cultured smooth muscle cells derived from Rhesus monkey thoracic aorta. Biochemistry 17:446–452

  21. McClain LD, Tomana M, Acton RT (1978) Purification and characterization of mouse brain Thy-1.2 differentiation alloantigen. Brain Res 159:161–171

  22. McLean IW, Nakane PK (1974) Periodate-lysine-paraformaldehyde fixative. A new fixative for immunoelectron microscopy. J Histochem Cytochem 22:1077–1083

  23. Morris RJ, Williams AF (1975) Antigens on mouse and rat lymphocytes recognized by rabbit antiserum against rat brain: the quantitative analysis of a xenogeneic antiserum. Eur J Immunol 5:274–281

  24. Morris RJ, Williams AF (1977) Serological and biochemical characteristics of a T lymphocyte differentiation antigen detected by rabbit antiserum to rat thymocyte membrane. Eur J Immunol 7:360–366

  25. Morris RJ, Mancini PE, Pfeiffer SE (1980) Thy-1 cell surface antigen on cloned nerve cell lines of the rat and mouse: amount, location and origin of the antigen on the cells. Brain Res 182:119–135

  26. Mosesson MW, Umfleet RA (1970) The cold insoluble globulin of human plasma. I. Purification, primary characterization and relationship to fibrinogen and other cold insoluble fraction components. J Biol Chem 245:5728–5736

  27. Nowack H, Gay S, Wick G, Becker U, Timpl R (1976) Preparation and use in immunohistology of antibodies specific for Type I and Type III collagen and procollagen. J Immunol Methods 12:117–124

  28. Pfeiffer SE, Betschart B, Cook J, Mancini P, Morris RJ (1978) Glial cell lines. In: Cell, tissue and organ culture in neurobiology, (S. Federoffand L. Hertz, eds.) Academic Press, pp 287–346 New York

  29. Raedler A, Arndt R, Raedler E, Jablonski D, Thiele H-G (1978) Evidence for the presence of Thy-1 on cultured thymic epithelial cells of mice and rats. Eur J Immunol 8:728–730

  30. Reif AE, Allen JMV (1964) The AKR thymic antigen and its distribution in leukaemias and nervous tissues. J Exp Med 120:413–433

  31. Ritter MA, Morris RJ (1980) Thy-1 antigen: selective association in lymphoid organs with the vascular basement membrane involved in lymphocyte recirculation. Immunology 39:85–91

  32. Ritter MA, Gordon L, Goldschneider I (1978) Distribution and identity of Thy-1 bearing cells during ontogeny in rat hemopoietic and lymphoid tissues. J Immunol 121:2463–2471

  33. Ritter MA, Morris RJ, Goldschneider I Immunology (1980 in press) Hidden Thy-1 antigen in a subpopulation of mouse bone marrow cells.

  34. Schachner M, Schoonmaker G, Hynes RO (1978) Cellular and subcellular localization of LETS protein in the nervous system. Brain Res 158:149–158

  35. Scheid MP, Boyse EA, Carswell EA, Old LJ (1972) Serologically demonstrable alloantigens of mouse epidermal cells. J Exp Med 135:938–955

  36. Shortman K, Williams N, Adams P (1972) The separation of different cell classes from lymphoid organs. V. Simple procedures for removal of cell debris, damaged cells and erythroid cells from lymphoid cell suspensions. J Immunol Methods 1:273–287

  37. Snodgrass MJ (1977) Ultrastructural distinction between reticular and collagenous fibres with an ammoniacal silver stain. Anat Rec 187:191–206

  38. Stenman S, Vaheri A (1978) Distribution of a major connective tissue protein, fibronectin, in normal human tissues. J Exp Med 147:1054–1064

  39. Stenn KS, Madri JA, Roll FJ (1979) Migrating epidermis produces AB2 collagen and requires continual collagen synthesis for movement. Nature 277:229–232

  40. Timpl R, Wick G, Gay S (1977) Antibodies to distinct types of collagens and procollagens and their applications in immunohistology. J Immunol Methods 18:165–182

  41. Trowbridge IS, Hyman R, Mazauskas C (1978a) The synthesis and properties of T25 glycoprotein in Thy-1-negative mutant lymphoma cells, Cell 14:21–32

  42. Trowbridge IS, Hyman R, Ferson T, Mazauskas C (1978b) Expression of Thy-1 glycoprotein on lectin-resistant lymphoma cell lines. Eur J Immunol 8:716–723

  43. Williams AF, Barclay AN, Letarte-Muirhead M, Morris RJ (1977) Rat Thy-1 antigens from thymus and brain: their tissue distribution, purification and chemical composition. Cold Spring Harbor Symp Quant Biol 41:51–61

  44. Yamada KM, Olden K (1978) Fibronectins-adhesive glycoproteins of cell surface and blood. Nature 275:179–184

Download references

Author information

Correspondence to Dr. Roger J. Morris.

Additional information

Part of this work was supported by grant no. NS10861 from the National Institute of Health and no. BNS 77-15818 from the National Science Foundation, awarded to Dr. S.E. Pfeiffer

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Morris, R.J., Ritter, M.A. Association of Thy-1 cell surface differentiation antigen with certain connective tissues in vivo. Cell Tissue Res. 206, 459–475 (1980).

Download citation

Key words

  • Antigens
  • Cell surface
  • Connective tissue
  • Collagen
  • Cell membrane proteins
  • Morphogenesis