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Lactate sensitive cells in newt kidneys

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Specimens of Triturus cristatus carnifex (Laurenti), raised in 3.2 g/l solution of sodium lactate, presented hypertrophy of “lactate sensitive cells” (LSC) clustered at the vascular pole of each renal corpuscle. As seen under both the light and electron microscopes, these are specialized cells of the Bowman's capsule located at the junction between its visceral and parietal layers. The structure formed by the cells, heretofore unknown, is topographically associated with the juxtaglomerular apparatus. As lactate stimulates erythropoiesis even in normal oxygenated specimens and hypoxia induces hypertrophy of LSC in newts, it is hypothesized that the LSC are the site of erythropoietin (ESF) synthesis. A scheme of ESF biogenesis in newts, integrating the above principles, concludes the paper.

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  1. Bellocci M, Picardi R, de Martino C (1971) The juxtaglomerular apparatus in the mesonephros of newt (Triturus cristatus). A morphologic study. Z Zellforsch 114:203–219

  2. Bowie DJ (1935–1936) A method for staining the pepsinogen granules in gastric glands. Anat Rec 64:357–367

  3. Busuttil RW, Roh BL, Fisher JW (1971) The cytological localization of erythropoietin in the human kidney using the fluorescent antibody technique. Proc Soc Exp Biol Med 137:327–330

  4. Busuttil RW, Roh BL, Fisher JW (1972) Localization of erythropoietin in the glomerulus of the hypoxic dog kidney using a fluorescent antibody technique. Acta Haemal 47:238–242

  5. Erslev AJ (1974) In vitro production of erythropoietin by kidneys perfused with a serum-free solution. Blood 44:77–85

  6. Fisher JW, Taylor G, Porteous D (1965) Localization of erythropoietin in glomeruli of sheep kidney by fluorescent antibody technique. Nature (London) 205:611–612

  7. Fisher JW, Busuttil RL, Roh BL (1970) Fluorescent antibody localization of erythropoietin in the glomerular tuft of the kidney. In: Matoth Y (ed) Erythropoiesis. Regulatory mechanisms and developmental aspects. Academic Press, New York London

  8. Frangioni G (1972) Ricerca comparativa sull'anemia sperimentale da acetil-fenil-idrazina (A.F.I.) e monossido di carbonio (CO) in Triturus cristatus carnifex. Boll Zool 39:617

  9. Frangioni G, Borgioli G (1978) The fundamental role of lactate in erythropoiesis: experimental studies on larvae of Amphibia Anura. Monitore Zool Ital (NS) 12:181–197

  10. Frangioni G, Borgioli, G (1979) Polystyrene embedding: a new method for light and electron microscopy. Stain Technol 54:167–172

  11. Frangioni G, Delfino G, Varlani C (1972) Anemia sperimentale in Triturus cristatus carnifex. Boll Soc Ital Biol Sper 48:1039–1042

  12. Frangioni G, Borgioli G, Delfino G (1979) Demonstration of the presence of macula densa in the nephrons of the newt by stimulation with sodium lactate. The problem of its physiological role. Monitore Zool Ital (NS) 13:25–43

  13. Frenkel ED, Suki W, Baum J (1968) Some observations on the localization of erythropoietin. Ann NY Acad Sci 149:292–293

  14. Gordon AS, Zanjani ED (1970) Mechanism of erythropoietin production. In: Matoth Y (ed) Erythropoiesis. Regulatory mechanisms and developmental aspects. Academic Press, New York London

  15. Gordon AS, Zanjani ED (1971) Studies on the renal erythropoietic factor (REF). In: Fisher JW (ed) Kidney hormones. Academic Press, New York London

  16. Gordon AS, Zanjani ED, McLaurin WD (1971) The renal erythropoietic factor (REF or erythrogenin). In: Fisher JW, Cafruny EJ (eds) Renal pharmacology. Appleton Century Crofts, New York

  17. Gordon AS, Zanjani ED, Gidari AS, Kuna RA (1973) Erythropoietin: the humoral regulator of erythropoiesis. In: Lobue J, Gordon AS (eds) Humoral control of growth and differentiation. Vol I. Vertebrate regulatory factors. Academic Press, New York London

  18. Harris JW, Kellermeyer RW (1972) The red cell. Production, metabolism, destruction: normal and abnormal. Harward University Press, Cambridge Massachusetts

  19. Huckabee WE (1958) Relationships of pyruvate and lactate during anaerobic metabolism. III. Effect on breathing low oxygen gases. J Clin Invest 37:264–271

  20. Keighley G, Lowy PH (1971) Chemistry and purification of erythropoietin. In: Fisher JW (ed) Kidney hormones. Academic Press, London New York

  21. Krantz SB, Jacobson LO (1970) Erythropoietin and the regulation of erythropoiesis. University of Chicago Press, Chicago London

  22. Kuratowska Z (1968) The renal mechanism of the formation and inactivation of erythropoietin. Ann N Y Acad Sci 149:128–134

  23. Kuratowska Z, Lewartowski B, Lipìnski B (1964) Chemical and biological properties of an erythropoietin-generating substance obtained from perfusates of isolated anoxic kidneys. J Lab Clin Med 64:226–237

  24. Miller ME, Howard D, Stohlman F, Flanagan P (1973) Mechanism of erythropoietin (Ep) production by cobalt (CoCl2). Abstr Am Soc Hematol 16:37

  25. Miller ME, Howard D, Stohlman F, Flanagan P (1974) Mechanism of erythropoietin production by cobaltous chloride. Blood 44:339–346

  26. Rodgers GM, George WJ, Fisher JW (1972) Increased kidney cyclic AMP levels and erythropoietin production following cobalt administration. Proc Soc Exp Biol Med 140:977–981

  27. Rodgers GM, Fisher JW, George WJ (1974) Lactate stimulation of renal cortical adenylate cyclase: a mechanism for erythropoietin production following cobalt treatment or hypoxia. J Pharmacol Exp Ther 190:542–550

  28. Sherwood JB, Goldwasser E (1978) Extraction of erythropoietin from normal kidneys. Endocrinology 103:866–870

  29. Sokabe H, Ogawa M (1974) Comparative studies of the juxtaglomerular apparatus. In: Bourne GH, Danielli JF (eds) International Review of Cytology. Academic Press, New York London, Vol 37:271–327

  30. Tepperman J, Tepperman HM (1948) On the blood lactic acid response to measured exercise in hypoxic human subjects. J Clin Invest 27:176–186

  31. Wilson W (1952) A new staining method for demonstrating the granules of the juxtaglomerular complex. Anat Rec 112:497–507

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Correspondence to Prof. Giuliano Frangioni.

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Frangioni, G., Borgioli, G. Lactate sensitive cells in newt kidneys. Cell Tissue Res. 207, 233–239 (1980). https://doi.org/10.1007/BF00237808

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Key words

  • Kidney
  • Triturus cristatus carnifex
  • Erythropoiesis
  • Electron microscopy